首页|Moxibustion of Zusanli(ST36)and Shenshu(BL23)alleviates the inflammation of rheumatoid arthritis in rats through regulating macrophage migration inhibitory factor/glucocorticoids signaling

Moxibustion of Zusanli(ST36)and Shenshu(BL23)alleviates the inflammation of rheumatoid arthritis in rats through regulating macrophage migration inhibitory factor/glucocorticoids signaling

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OBJECTIVE:To test the hypothesis that moxibustion may inhibit rheumatoid arthritis(RA)synovial inflammation by regulating the expression of macrophage migration inhibitory factor(MIF)/glucocorticoids(GCs).METHODS:Fifty male Sprague-Dawley rats were randomly divided into five groups(n=10 each):blank Control(CON)group,RA Model(RA)group,Moxibustion(MOX)group,MIF inhibitor(S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester(ISO-1)group,and Moxibustion+MIF inhibitor ISO-1(MOX+ISO-1)group.Rats in the ISO-1 group and ISO-1+MOX group were intraperitoneally injected with the inhibitor ISO-1.The rats in the RA group,ISO-1 group,MOX group,and ISO-1+MOX group were injected with Freund's complete adjuvant(FCA)in the right hind footpad to establish an experimental RA rat model.In the MOX group and MOX+ISO-1 group,rats were treated with Moxa.The thickness of the footpads of the rats in each group was measured at three-time points before,after modeling and after moxibustion treatment.The contents of serum MIF,corticosterone(CORT),tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were detected by enzyme-linked immunosorbent assay;and the contents of synovial MIF were detected by Western blot.Hematoxylin-eosin(HE)staining method was used to observe the pathological changes of synovial tissue under a section light microscope,and pathological scoring was performed according to the grading standard of the degree of synovial tissue disease.RESULTS:Moxibustion was found to reduce the level of MIF and alleviate inflammation in RA rats in this study.In addition,after inhibiting the expression of MIF,the level of CORT increased,and the level of TNF-α decreased.Treating RA rats with inhibited MIF by moxibustion,the level of CORT was almost unchanged,but the level of TNF-α further decreased.The correlation analysis data suggested that MIF was positively related to the expression of TNF-α and negatively correlated with the expression of CORT.CONCLUSION:Reducing MIF to increase CORT and decrease TNF-α by moxibustion treatment in RA.MIF may be a factor for moxibustion to regulate the expression of CORT,but the expression of TNF-α is due to the incomplete regulation of the MIF.This study added to the body of evidence pointing to moxibustion's anti-inflammatory mechanism in the treatment of RA.

rheumatoid arthritismacrophage migration inhibitory factormoxibustioncorticosteroneglucocorticoids

ZHANG Linlin、ZHONG Yumei、LU Wenting、SHANG Yanan、GUO Yanding、LUO Xiaochao、CHEN Yang、LUO Kun、HU Danhui、YU Huiling、ZHOU Haiyan

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Acupuncture and Moxibustion College,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China

Department of Painology,First People's Hospital of Chengdu,Chengdu 610095,China

External treatment center,First People's Hospital of Chengdu,Chengdu 610095,China

Chinese Evidence-Based Medicine Centre,West China Hospital,Sichuan University,Chengdu 610044,China

Traditional Chinese Medicine College,Chongqing Medical University,Chongqing 400016,China

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National Key R&D Program of ChinaNational Natural Science Foundation of China&&Foundation of Sichuan Provincial Administration of Traditional Chinese MedicineScience and Technology Innovation Seedling Project of Sichuan Province,ChinaChengdu University of Traditional Chinese Medicine Foundation

2019YFC170900181973959817744352018JC0072022037QNXZ2018034

2024

10.19852/j.cnki.jtcm.20220602.001

中医杂志(英文版)
中国中医药学会 中国中医研究院

中医杂志(英文版)

影响因子：0.855
ISSN：0255-2922
年,卷(期)：2024.44(2)
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