首页|Huoxue Jiedu Huayu recipe(活血解方)inhibits macrophage-secreted vascular endothelial growth factor-a on angiogenesis and alleviates renal fibrosis in the contralateral kidneys of unilateral ureteral obstruction rats

Huoxue Jiedu Huayu recipe(活血解方)inhibits macrophage-secreted vascular endothelial growth factor-a on angiogenesis and alleviates renal fibrosis in the contralateral kidneys of unilateral ureteral obstruction rats

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OBJECTIVE:To elucidate the mechanism by which Huoxue Jiedu Huayu recipe(活血解毒化瘀方,HJHR)regulates angiogenesis in the contralateral kidney of unilateral ureteral obstruction(UUO)rats and the mechanism by which it reduces of renal fibrosis.METHODS:Male Wistar rats were randomly divided into 4 groups:the sham group,UUO group(180 d of left ureter ligation),UUO plus eplerenone(EPL)group,and UUO plus HJHR group.After 180 d of oral drug administration,blood and contralateral kidneys were collected for analysis.Angiogenesis-and fibrosis-related indexes were detected.RESULTS:HJHR and EPL improved structural damage and renal interstitial fibrosis in the contralateral kidney and reduced the protein expression levels of α-smooth muscle actin(α-SMA),vimentin and collagen I.Moreover,these treatments could reduce the expression of vascular endothelial growth factor-A(VEGFA)by inhibiting the infiltration of macrophages.Furthermore,HJHR and EPL significantly reduced the expression of CD34 and CD105 by downregulating VEGFA production,which inhibited angiogenesis.Finally,the coexpressions of CD34,CD105 and α-SMA were decreased in the HJHR and EPL groups,indicating that endothelial-to-mesenchymal transition was inhibited.CONCLUSIONS:These findings confirm that HJHR alleviates contralateral renal fibrosis by inhibiting VEGFA-induced angiogenesis,encourage the use of HJHR against renal interstitial fibrosis and provide a theoretical basis for the clinical management of patients with CKD.

epithelial-mesenchymal transitionaldosteroneeplerenonereceptors,mineralocorticoidunilateral ureteral obstructionHuoxue Jiedu Huayu recipe

GAO Xiaomeng、QIANG Panpan、CHANG Jingyue、FAN Lili、YANG Fan、XU Qingyou

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Graduate School,Hebei University of Chinese Medicine

Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns,Hebei University of Chinese Medicine,Shijiazhuang 050091,China

Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns,Hebei University of Chinese Medicine

Institute of Integrative Medicine,Hebei University of Chinese Medicine,Shijiazhuang 050091,China

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Natural Science Foundationfunded ProjectNatural Science Foundationfunded ProjectHebei Provincial Postgraduate Innovative Ability Cultivation Funding ProjectConstruction Program of New Research and Development Platform and Institution,Hebei Province Innovation Ability Promotion Plan

8187325182174317CXZZBS202314020567624H

2024

10.19852/j.cnki.jtcm.20240423.005

中医杂志(英文版)
中国中医药学会 中国中医研究院

中医杂志(英文版)

影响因子：0.855
ISSN：0255-2922
年,卷(期)：2024.44(3)
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