首页|Investigation of the active ingredients and mechanism of Shuangling extract in dextran sulfate sodium salt induced ulcerative colitis mice based on network pharmacology and experimental verification

Investigation of the active ingredients and mechanism of Shuangling extract in dextran sulfate sodium salt induced ulcerative colitis mice based on network pharmacology and experimental verification

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OBJECTIVE:To explore the pharmacodynamic effects and potential mechanisms of Shuangling extract against ulcerative colitis(UC).METHODS:The bioinformatics method was used to predict the active ingredients and action targets of Shuangling extract against UC in mice.And the biological experiments such as serum biochemical indexes and histopathological staining were used to verify the pharmacological effect and mechanism of Shuangling extract against UC in mice.RESULTS:The Shuangling extract reduced the levels of seruminterleukin-1β(IL-1β),tumor necrosis factor-a(TNF-N),interleukin-6(IL-6)and other inflammatory factors in UC mice and inhibited the inflammatory response.AKT Serine/threonine Kinase 1 and IL-6 may be the main targets of the anti-UC action of Shuangling extract,and the TNF signaling pathway,Forkhead box O signaling pathway and T-cell receptor signaling pathway may be the main signaling pathways.CONCLUSION:The Shuangling extract could inhibit the inflammatory response induced by UC and regulate intestinal immune function through multiple targets and multiple channels,which provided a new option and theoretical basis for anti-UC.

colitis,ulcerativepharmacodynamic studynetwork pharmacologymolecular docking simulationShuangling extract

CHU Mengzhen、WANG Yu、LIN Zhijian、Lyu Jintao、ZHANG Xiaomeng、ZHANG Bing

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Department of Clinical Chinese Pharmacy,School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China

Innovation Team and Talents Support Program of National Administration of Traditional Chinese MedicineNational Leading Talents Support Plan of Traditional Chinese Medicine'Qihuang Scholar'Plan

ZYYCXTD-C-202005-111040063320004

2024

10.19852/j.cnki.jtcm.20240408.003

中医杂志(英文版)
中国中医药学会 中国中医研究院

中医杂志(英文版)

影响因子：0.855
ISSN：0255-2922
年,卷(期)：2024.44(3)
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