Abstract
OBJECTIVE:To investigate the effects of Hippeastrum hybridum(HH)as a free radical scavenger,and an inhibitor of the two enzymes i-e Alpha-amylase(α-amylase)and acetylcholinesterase(AChE).METHODS:In this study,HH plant was preliminary analyzed for phytochemical screening and then tested for its antioxidant,anti-α-amylase,and anti-AChE efficiency via standard procedures.RESULTS:Phytochemical analysis shows the existence of different compounds;while Coumarins and quinones were absent.The total phenolic,flavonoid,and tannins content were found to be(78.52±0.69)mg GAE/g,(2.01±0.04)mg RUE/g,and(58.12±0.23)mg TAE/g of plant extract respectively.28.02％±0.02％alkaloid and 2.02％±0.05％saponins were present in the HH extract.The HH extract showed the anti-oxidant property with IC50(50％inhibition)of(151.01±0.13)(HH),(79.01±0.04)(Ascorbic acid)for ferric reducing,(91.48±0.13)(HH),(48.02±0.11)(Ascorbic acid)against Ammonium molybdenum,(156.02±0.31)(HH),(52.38±0.21)(Ascorbic acid)against DPPH,136.01±0.21(HH),52.02±0.31(Ascorbic acid)against H2O2,and 154.12±0.03(HH),(40.05±0.15)(Ascorbic acid)μg/mL against ABTS respectively.Statistical analysis indicated that HH caused a competitive type of inhibition of α-amylase(Vmax remained constant and Km increases from 10.65 to 84.37％)while Glucophage caused the un-competitive type of inhibition i-e both Km and Vmax decreased from 40.49 to 69.15％and 38.86 to 69.61％respectively.The Ki,(inhibition constant);Kl,(dissociation constant),Km,(Michaelis-Menten constant),and IC50 were found to be 62,364,68.1,and 38.08±0.22 for HH and 12,101.05,195,34.01±0.21 for Glucophage.Similarly,HH causes an anon-competitive type of inhibition of AChE i-e Km remains constant while Vmax decreases from 60.5％to 74.1％.The calculated Ki,Kl,Km,and IC50 were found to be 32,36.2,0.05,and 18.117±0.018.CONCLUSION:From the current results,it is concluded that HH extract contains bioactive compounds,and could be a good alternative to controlling oxidants,Alzheimer's and Type-Ⅱ diabetic diseases.
基金项目
Higher Education Commission of Pakistan(HEC-PAK)(20-5082/NRPU/R&D/HEC)
NETL
NSTL
万方数据