Saponin Ⅰ from Shuitianqi(Rhizoma Schizocapasae Plantagineae)inhibits metastasis by negatively regulating the transforming growth factor-β1/Smad7 network and epithelial-mesenchymal transition in the intrahepatic metastasis Bagg's Albino/c mouse model

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外文标题：Saponin Ⅰ from Shuitianqi(Rhizoma Schizocapasae Plantagineae)inhibits metastasis by negatively regulating the transforming growth factor-β1/Smad7 network and epithelial-mesenchymal transition in the intrahepatic metastasis Bagg's Albino/c mouse model

外文摘要：OBJECTIVE:To examine the influence of Saponin Ⅰ from Shuiti anqi(Rhizoma Schizocapasae Plantagineae)(SSPH Ⅰ)on hepatocellular carcinoma(HCC)metastasis,and to elucidate the underlying mechanism.METHODS:The intrahepatic metastasis Bagg's Albino/c(BALB/c)mouse model was established with human hepatocellular carcinomas(HepG2)cells,then treated with normal saline(once per day),cisplatin(2 mg/kg,once every 2 d),and SSPH Ⅰ(25,50,and 75 mg/kg,once per day).Then,we assessed alterations in the hepatic pathology and target protein expressions in the intrahepatic metastasis BALB/c mouse model using a series of molecular biology techniques.RESULTS:Based on our analysis,SSPH Ⅰ significantly alleviated hepatocyte necrosis and tumor cells infiltration.Moreover,SSPH Ⅰ suppressed extracellular matrix(ECM)degradation and angiogenesis via a decrease in matrix etalloproteinase-2(MMP-2),MMP-9,CD31,CD34,and vascular endothelial growth factor(VEGF)levels.Furthermore,SSPH Ⅰ repressed invasion and meta-stasis by suppressing the transforming growth factor-β 1(TGF-β 1)/Smad7 axis and epithelial-mesenchymal transition(EMT),as evidenced by the scarce TGF-β1,N-cadherin,and Vimentin expressions,and elevated Smad7 and E-cadherin expressions.CONCLUSION:The SSPH Ⅰ-mediated negative regulation of the TGF-β 1/Smad7 axis and EMT are critical for the inhibition of HCC invasion and metastasis.

外文关键词：

carcinoma,hepatocellularepithelial-mesenchymal transitiontransforming growth factor beta1Smad7 proteinSaponin Ⅰ from Shuitianqi(Rhizoma Schizocapasae Plantagineae)

作者：

LYU Meixian、ZHOU Huan、ZHI Limin、ZHOU Jinling、GAN Rizhi、QIN Yanping、HE Nengting、ZUO Qiqi、LI Hao、DONG Min、LIANG Gang

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作者单位：

Pharmaceutical College,Guangxi Medical University,Nanning 530022,China

Department of Pharmacy,Affiliated Tumor Hospital of Guangxi Medical University,Nanning 530022,China

Drug clinical trial Facility office,the People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530022,China

College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530022,China

Department of Pharmacy,the Second Affiliated Hospital of Guangxi Medical University,Nanning 530005,China

Department of Pharmacy,People's Hospital of Guangxi Hezhou,Hezhou 542899,China

Department of Pharmacy,Guangzhou Women and Children's Medical Center Liuzhou Hospital,Liuzhou 545000,China

Department of Pharmacy,First Affiliated Hospital of Gannan Medical University,Gannan 341000,China

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关键词：

carcinoma,hepatocellular epithelial-mesenchymal transition transforming growth factor beta1 Smad7 protein Saponin Ⅰ from Shuitianqi(Rhizoma Schizocapasae Plantagineae)

出版年：

2024

DOI：

10.19852/j.cnki.jtcm.2024.04.006

中医杂志(英文版)

中国中医药学会中国中医研究院

中医杂志(英文版)

影响因子：0.855

ISSN：0255-2922

年,卷(期)：2024.44(4)