首页|Adenosine triphosphate mediates the pain tolerance effect of manual acupuncture at Zusanli(ST36)in mice

Adenosine triphosphate mediates the pain tolerance effect of manual acupuncture at Zusanli(ST36)in mice

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Adenosine triphosphate mediates the pain tolerance effect of manual acupuncture at Zusanli(ST36)in mice
OBJECTIVE:To investigate the mechanisms behind the effects of acupuncture in Traditional Chinese Medicine,we delved into the adenosine triphosphate/peripheral purinergic P2X receptor 3(ATP/P2X3)receptor signaling system as an indicator of the body's energy state,commonly referred to as"Qi'.METHODS:The tail-flick test was utilized to explore the impact of acupuncture on pain tolerance threshold(PTT)in mice,while also assessing adenosine(ADO)levels and adenylate energy charge(EC)at Zusanli(ST36).The study further investigated the dose-dependent effects of acupuncture on PTT and ADO levels at Zusanli(ST36).To shed light on the underlying mechanisms of acupuncture's effects,the study examined the impact of ATP,a P2X3 receptor antagonist,and adenosine disodium on PTT following acupuncture administration.RESULTS:Acupuncture at Zusanli(ST36)led to significant improvements in PTT in mice,with the most effective interventions being twirling for 2 min and needle retention for 28 min.These interventions also resulted in significant increases in ATP levels.The effects of acupuncture were further augmented by administration of different doses of ATP at Zusanli(ST36),and pretreatment with a P2X3 receptor antagonist decreased PTT.Adenylate EC peaked at 30 min after intraperitoneal injection of ATP,and pretreatment with various doses of i.p.ATP 30 min prior to acupuncture increased PTT in a dose-dependent manner.Additionally,pretreatment with an i.p.or intramuscular injection of adenosine disodium enhanced the effects of acupuncture.CONCLUSION:This research provides compelling evidence that ATP is involved in the regulation of PTT through acupuncture,revealing new avenues for achieving enhanced clinical outcomes.

acupuncturepoint ST36(Zusanli)adenosine triphosphatereceptors,purinergic P2X3energy state

LI Zhongzheng、ZHAO Yadan、Ma Weigang、Zhang Yonglong、XU Zhifang、XI Qiang、LI Yanqi、QIN Siru、ZHANG Zichen、WANG Songtao、ZHAO Xue、LIU Yangyang、GUO Yi、GUO Yongming

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Research Center of Experimental Acupuncture Science,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China

National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 301617,China

Department of Acupuncture and Moxibustion,Qingdao Hiser Hospital Affiliated of Qingdao University(Qingdao Traditional Chinese Medicine Hospital),Qingdao 266033,China

School of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China

School of Acupuncture and Moxibustion and Tuina,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China

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acupuncture point ST36(Zusanli) adenosine triphosphate receptors,purinergic P2X3 energy state

2024

10.19852/j.cnki.jtcm.20240626.003

中医杂志(英文版)
中国中医药学会 中国中医研究院

中医杂志(英文版)

影响因子：0.855
ISSN：0255-2922
年,卷(期)：2024.44(4)