Effect on serum metabolomics of rats with premature ovarian insufficiency by Zhibian(BL54)through Shuidao(ST28)acupuncture

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外文标题：Effect on serum metabolomics of rats with premature ovarian insufficiency by Zhibian(BL54)through Shuidao(ST28)acupuncture

外文摘要：OBJECTIVE:To analyze the serum metabolic targets of the"Zhibian(BL54)through Shuidao(ST28)"acupuncture technique in cyclophosphamide(CTX)-induced premature ovarian insufficiency(POI)model rats and to elucidate the potential molecular mechanism of acupuncture in improving POI.METHODS:We used an intraperitoneal injection of CTX to establish the POI rat model(POI group)and compared serum hormone levels and ovarian histopathological changes to evaluate the effect of the Zhibian(BL54)through Shuidao(ST28)technique(ZS+POI group)on ovarian function.Then,nontargeted metabolomics was performed using rat serum by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS).RESULTS:After acupuncture intervention,the serum hormone levels and ovarian pathological morphology of POI rats were effectively improved.Moreover,UPLC-Q-TOF/MS results showed that the ZS+POI group showed a significant reversal of the levels of 6 differential metabolites.Among them,the levels of four serum metabolic markers,divanillyltetrahydrofuran ferulate,trans-ferulic acid,tryptamine,and neuraminic acid,increased significantly.Further analysis of biological effects showed that all metabolites were involved in the regulation of reproductive hormone levels and antioxidant and antiapoptotic effects.CONCLUSIONS:The"Zhibian(BL54)through Shuidao(ST28)"acupuncture method may improve the ovarian function of POI rats by regulating serum metabolite markers to exert antioxidant and antiapoptotic effects,which provides a theoretical basis for the clinical application of acupuncture in the treatment of POI.

外文关键词：

acupuncturepremature ovarian insufficiencymetabolomicsultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometryZhibian(BL54)through Shuidao(ST28)

作者：

YAN Jing、FENG Huimin、QIU Fang、WANG Haijun、YIN Luyun、JIN Xiaofei、ZHAO Jiyu、WANG Hongyang、YAN Xiaoqin

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作者单位：

Second Clinical College of Shanxi University of Traditional Chinese Medicine,Jinzhong 030619,China

Research Center of Experimental Acupuncture Science,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China

Department of Cervical and Lumbar Vertebral Diseases,Jinzhong Hospital Affiliated to Shanxi University of Traditional Chinese Medicine,Jinzhong 030600,China

关键词：

acupuncture premature ovarian insufficiency metabolomics ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry Zhibian(BL54)through Shuidao(ST28)

出版年：

2024

DOI：

10.19852/j.cnki.jtcm.20231226.001

中医杂志(英文版)

中国中医药学会中国中医研究院

中医杂志(英文版)

影响因子：0.855

ISSN：0255-2922

年,卷(期)：2024.44(4)