首页|Actinidia chinensis polysaccharide interferes with the epithelial-mesenchymal transition of gastric cancer by regulating the nuclear transcription factor-KB pathway to inhibit invasion and metastasis

Actinidia chinensis polysaccharide interferes with the epithelial-mesenchymal transition of gastric cancer by regulating the nuclear transcription factor-KB pathway to inhibit invasion and metastasis

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OBJECTIVE:To investigate the mechanisms of the effect of Actinidia chinensis polysaccharide(ACPS)on the invasion and metastasis of gastric cancer cells.METHODS:BGC-823-Luc gastric cancer cells stably transfected with a luciferase gene were used to establish an insitutransplanted tumor mouse model.A live mouse imaging system was used to observe tumor growth,and hematoxylin and eosin staining was applied to analyze tissue histopathology.Transwell and scratch wound assays were performed to examine the invasive and migratory ability of BGC-823 cells.Immunofluorescence,confocal microscopy,immunohistochemistry,and Western blot assays were used to analyze the expressions of the nuclear transcription factor-κB(NF-κB)signaling pathway and epithelial-mesenchymal transition(EMT)-related proteins.RESULTS:ACPS significantly inhibited the growth of subcutaneously transplanted BGC-823-Luc gastric cancer tumors in nude mice and reduced inflammatory cell infiltration in tumor tissues.ACPS inhibited Epidermal Growth Factor-induced invasion,migration,and morphological changes in the cytoskeleton of BGC-823 cells.ACPS inhibited gastric cancer EMT and decreased the expression of matrix metallopeptidase 9,N-cadherin and p-NF-κB p65 in transplanted tumor tissues.ACPS inhibited the expression of matrix metalloproteinases and vascular adhesion factors in BGC-823 cells,promoted p65-NF-κB nuclear translocation,and regulated proteins associated with the NF-κB p65 pathway.CONCLUSIONS:ACPS inhibited gastric cancer invasion and metastasis both in vivo and in vitro,which evidenced the inhibition of gastric cancer EMT via regulating the NF-κB inflammatory pathway.

stomach neoplasmsinvasionneoplasm metastasisepithelial-mesenchymal transitionNF-kappa Bsignal transductionActinidia chinensis polysaccharide

ZHANG Guangshun、XU Xiaonan、XU Chuyun、LIAO Guanghui、XU Hao、LOU Zhaohuan、ZHANG Guangji

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School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China

Key Labortary of Blood-stasis-toxin Syndrome of Zhejiang Province,Hangzhou 310053,China

School of Basic Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China

Traditional Chinese Medicine"Preventing Disease"Wisdom Health Project Research Center of Zhejiang,Hangzhou 310053,China

the First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310053,China

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2024

10.19852/j.cnki.jtcm.20240806.001

中医杂志(英文版)
中国中医药学会 中国中医研究院

中医杂志(英文版)

影响因子：0.855
ISSN：0255-2922
年,卷(期)：2024.44(5)