首页|Huaiyu pill(槐榆片)alleviates inflammatory bowel disease in mice via blocking toll like receptor 4/myeloid differentiation primary response gene 88/nuclear factor kappa B subunit 1 pathway
Huaiyu pill(槐榆片)alleviates inflammatory bowel disease in mice via blocking toll like receptor 4/myeloid differentiation primary response gene 88/nuclear factor kappa B subunit 1 pathway
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Huaiyu pill(槐榆片)alleviates inflammatory bowel disease in mice via blocking toll like receptor 4/myeloid differentiation primary response gene 88/nuclear factor kappa B subunit 1 pathway
OBJECTIVE:To investigate the therapeutic effects of Huaiyu pill(槐榆片,HYP)on inflammatory bowel disease(IBD)and the underlying mechanisms have not been elucidated.METHODS:To establish the IBD model,mice were administered with dextran sulfate sodium(DSS).Mice were intragastrically pre-treated with sulfasalazine(SASP)and HYP.Disease activity index(DAI)and colon length were monitored,and the colonic tissues were subjected to hematoxylin-eosin staining.Pro-inflammatory factors and vascular inflammation-related proteins were determined using enzyme-linked immunosorbent assay(ELISA).The potential mechanisms of HYP were examined using network pharmacology analysis.The expressions of zona occludens 1(ZO-1),occludin,toll like receptor 4(TLR4),myeloid differentiation primary response gene 88(MYD88),and nuclear factor kappa B p65 subunit(NF-κB p65)in colon tissues were examined using Western blotting or immunohistochemical analyses.RESULTS:Pre-treatment with HYP enhanced the colon length,decreased DAI scores,and mitigated histopathological alterations in DSS-treated mice.HYP alleviated intestinal inflammation by downregulating the levels of interleukin 1 beta(IL-1β),interleukin 6(IL-6),tumor necrosis factor alpha(TNF-α)and interleukin 17(IL-17).Additionally,HYP suppressed the disruption of the gut barrier by upregulating the ZO-1,occludin,and mucin 2(MUC2)levels and downregulating the endothelin 1(ET-1)and erythropoietin(EPO)levels.Network pharmacological analysis and experimental results revealed that HYP downregulated the colonic tissue levels of TLR4,MYD88,and NF-κB p65 in DSS-treated mice.CONCLUSION:This study investigated the in vivo therapeutic effects of HYP on IBD and the underlying molecular mechanisms.These findings provide an experimental foundation for the clinical application of HYP.
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