Multi-omics analysis reveals the neuroprotective effect of Atractylodis Rhizoma Alba extract against Parkinson's disease in mouse

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外文摘要：OBJECTIVE:To assess Atractylodis Rhizoma Alba extract(ARE)neuroprotective function in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-treated mice and related genes.METHODS:Examined mRNA-DNA methylation changes induced by ARE in MPTP-induced Parkinson's disease(PD)model's substantia nigra.RESULTS:ARE mitigated MPTP-induced motor impairment in rotarod and open field tests and preserved tyrosine hydroxylase-positive neuronal cells in substantia nigra and striatum.Genome RNA-Sequencing and Methyl-Sequencing in substantia nigra of vehicle/ARE-treated MPTP-induced PD mice showed 84 differentially expressed genes(DEGs)and 1804 differentially methylated regions(DMRs).Upregulated genes involved zinc ion homeostasis,cilium protein localization,and transcription;downregulated genes linked to ephrin receptor signaling,somitogenesis,and gene expression regulation.Hyper/hypomethylated DMRs post-ARE treatment associated with Wnt signaling,mitochondrial organization,dopamine biosynthesis,and hindbrain development.No significant correlation between DEGs and methylated genes related to PD pathogenesis.CONCLUSION:This research has identified the epigenetic targets of ARE's therapeutic action and gives insight on how ARE protects neurons in Parkinson's disease.

外文关键词：

Parkinson diseasenerve degeneration1-methyl-4-phenyl-1236-tetrahydropyridineAtractylodis Rhizoma AlbaMethyl-seq

作者：

KANG Sohi、LEE Sueun、MOON Byeong Cheol、SONG Jun Ho、KIM Sung-Ho、MOON Changjong、LEE Soong-In、KIM Chul、KIM Joong Sun

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作者单位：

Department of Anatomy and Convergence Medical Science,College of Medicine,Institute of Health Sciences,Gyeongsang National University,Jinju 52727,Republic of Korea

College of Veterinary Medicine and BK21 FOUR Program,Chonnam National University,Gwangju 61186,Republic of Korea

Herbal Medicine Resources Research Center,Korea Institute of Oriental Medicine,111,Geonjae-ro,Naju-si,Jeollanam-do 58245,Republic of Korea

Department of Biology,Chungbuk National University,Cheongju 28644,Republic of Korea

Department of Oriental Medicine Prescription,College of Oriental Medicine,Dong-Shin University,Naju-si,Jeollanam-do 58245,Republic of Korea

KM Data Division,Korea Institute of Oriental Medicine,1672 Yuseong-daero,Yuseong-gu,Daejeon 34054,Republic of Korea

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出版年：

2024

DOI：

10.19852/j.cnki.jtcm.2024.06.002

中医杂志(英文版)

中国中医药学会中国中医研究院

中医杂志(英文版)

影响因子：0.855

ISSN：0255-2922

年,卷(期)：2024.44(6)