Abstract
OBJECTIVE:To investigate the mechanism and effect of Renshen(Radix Ginseng)polysaccharide on the migration of intestinal epithelial cell line 6(IEC-6),as well as the repair mechanism of Renshen(Radix Ginseng)polysaccharide on colonic injury induced by dextran sulfate sodium(DSS)in mice.METHODS:Mice were fed 3％(w/v)DSS for 6 d to create colonic lesions.A cell-migration model was created using cell scratching.mRNA expression,protein expression,translation efficiency of mRNA,and nucleoplasmic distribution of human antigen R(HuR)were determined by real-time reverse transcription-quantitative polymerase chain reaction,western blotting,a dual luciferase reporter system,and immunofluorescence staining,respectively.RESULTS:Renshen(Radix Ginseng)polysaccharide promoted the migration of IEC-6 cells and affected expression of stromal interaction molecule 1(STIM1)and cell division cycle 42(Cdc42)at transcriptional and post-transcriptional levels.CONCLUSIONS:Renshen(Radix Ginseng)polysaccharide-induced repair of intestinal mucosal injury may be mediated by increased cell migration via polyamine-based regulatory mechanisms.In vitro and in vivo experiments suggest that Renshen(Radix Ginseng)polysaccharide-induced post-transcriptional regulation of STIM1 and Cdc42 may be related to differences in the regulation of different target genes by HuR.Taken together,these data provide a reference for further exploration of the protective effect of Renshen(Radix Ginseng)on the intestinal mucosa.
万方数据