首页|Bushen Huoxue decoction(补肾活血汤)improves the reproduction of endometriosis-associated infertility by regulating Homeobox A10 and α lpha(v)beta(3)integrin expression
Bushen Huoxue decoction(补肾活血汤)improves the reproduction of endometriosis-associated infertility by regulating Homeobox A10 and α lpha(v)beta(3)integrin expression
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Bushen Huoxue decoction(补肾活血汤)improves the reproduction of endometriosis-associated infertility by regulating Homeobox A10 and α lpha(v)beta(3)integrin expression
OBJECTIVE:To investigate the mechanism of Bushen Huoxue decoction(补肾活血汤,BSHXD)to treat endometriosis-induced infertility.MEDHODS:The main compounds of BSHXD were determined by high performance liquid chromatography-mass spectrometry(HPLC-MS/MS).The effect of BSHXD on Homeobox A10(HOXA10)and alpha(v)beta(3)(αvβ3)integrin expression of Ishikawa cells,mouse model,and endometriosis-associated infertility women was evaluated by using Western blot analysis,immunohistochemistry and Real-Time quantitative polymerase chain reaction(RT-qPCR).The efficacy of BSHXD on embryo attachment were examined by using the BeWo spheroid and mouse embryo attachment assay.HOXA10 concentration in uterine flushing fluid of endometriosis-associated infertility women treated with BSHXD was measured by Enzyme-Linked immunosorbent assay(ELISA).RESULTS:BSHXD improved BeWo spheroid and mice blastocysts attachment to Ishikawa cells and increased embryo implantation rates in mice and pregnancy rates in women with endometriosis-associated infertility.BSHXD enhanced HOXA10 and αvβ3 integrin expression in Ishikawa cell,endometriosis mouse model,and endometriosis-associated infertility women,which potentially improved endometrial receptivity.CONCLUSIONS:BSHXD could improve endometrial receptivity of endometriosis-associated infertility in a dose-dependent manner by regulating HOXA10 and αvβ3 integrin expression.
Department of Obstetrics and Gynecology,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China
Department of Pathology,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China
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