Abstract
OBJECTIVE:To investigate the mechanism of Bushen Huoxue decoction(补肾活血汤,BSHXD)to treat endometriosis-induced infertility.MEDHODS:The main compounds of BSHXD were determined by high performance liquid chromatography-mass spectrometry(HPLC-MS/MS).The effect of BSHXD on Homeobox A10(HOXA10)and alpha(v)beta(3)(αvβ3)integrin expression of Ishikawa cells,mouse model,and endometriosis-associated infertility women was evaluated by using Western blot analysis,immunohistochemistry and Real-Time quantitative polymerase chain reaction(RT-qPCR).The efficacy of BSHXD on embryo attachment were examined by using the BeWo spheroid and mouse embryo attachment assay.HOXA10 concentration in uterine flushing fluid of endometriosis-associated infertility women treated with BSHXD was measured by Enzyme-Linked immunosorbent assay(ELISA).RESULTS:BSHXD improved BeWo spheroid and mice blastocysts attachment to Ishikawa cells and increased embryo implantation rates in mice and pregnancy rates in women with endometriosis-associated infertility.BSHXD enhanced HOXA10 and αvβ3 integrin expression in Ishikawa cell,endometriosis mouse model,and endometriosis-associated infertility women,which potentially improved endometrial receptivity.CONCLUSIONS:BSHXD could improve endometrial receptivity of endometriosis-associated infertility in a dose-dependent manner by regulating HOXA10 and αvβ3 integrin expression.
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