首页|Anti-oxidative and immunological role of Cyclocarya paliurus polysaccharide on the liver injury of diabetic rats
Anti-oxidative and immunological role of Cyclocarya paliurus polysaccharide on the liver injury of diabetic rats
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
万方数据
维普
Anti-oxidative and immunological role of Cyclocarya paliurus polysaccharide on the liver injury of diabetic rats
OBJECTIVE:To investigate the effects of Cyclocarya paliurus(C.paliurus)polysaccharide on the liver injury of diabetic rats.METHODS:Rats were divided into 6 groups,including normal group,model group,control group,low-dose group of C.paliurus polysaccharide treatment,middle-dose group of C.paliurus polysaccharide treatment and high-dose group of C.paliurus polysaccharide treatment.Histological analysis of liver was analyzed using hematoxilin and eosin.Levels of plasma biological parameters and anti-oxidative enzymes were determined by spectrophotometry.Nuclear factor kappa-B-p65(NF-κB p65),tumor necrosis factor-α(TNF-α),interleukins 6(IL-6),IL-1β were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay.RESULTS:Compared with that of model group,the glucose level of plasma decreased 62.32％(P＜0.01),but glycogen and insulin level increased 1.51 times and 1.27 times in the high-dose group of C.paliurus polysaccharide treatment,respectively.Plasma alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)level decreased 47.47％(P＜0.05),43.65％(P＜0.05)and 50.51％(P＜0.05)in the high-dose group of C.paliurus polysaccharide treatment,respectively.Superoxide dismutase,catalase,glutathione peroxidase,glutathione reductase level increased 1.16 times(P＜0.01),71.28％(P＜0.05),1.29 times(P＜0.01)and 87.46％(P＜0.05)in the high-dose group of C.paliurus polysaccharide treatment,respectively.Enzyme activities of NF-κB,TNF-α,IL-1βand IL-6 were decreased 39.29％(P＜0.05),51.11％(P＜0.05),37.42％(P＜0.05)and 36.50％(P＜0.05),respectively.CONCLUSIONS:Administration of C.paliurus polysaccharide may play a protecting role for liver injury of diabetic rats through lowering glucose,ALT,AST,ALP level,increasing glycogen and insulin level,enhancing the anti-oxidative ability and down-regulating the inflammatory factors expression.
Basic Medicine College of Nanyang Medical University,Nanyang 473041,China
Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases,Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China
万方数据
维普
