Abstract
OBJECTIVE:To investigate the effects of Cyclocarya paliurus(C.paliurus)polysaccharide on the liver injury of diabetic rats.METHODS:Rats were divided into 6 groups,including normal group,model group,control group,low-dose group of C.paliurus polysaccharide treatment,middle-dose group of C.paliurus polysaccharide treatment and high-dose group of C.paliurus polysaccharide treatment.Histological analysis of liver was analyzed using hematoxilin and eosin.Levels of plasma biological parameters and anti-oxidative enzymes were determined by spectrophotometry.Nuclear factor kappa-B-p65(NF-κB p65),tumor necrosis factor-α(TNF-α),interleukins 6(IL-6),IL-1β were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay.RESULTS:Compared with that of model group,the glucose level of plasma decreased 62.32％(P＜0.01),but glycogen and insulin level increased 1.51 times and 1.27 times in the high-dose group of C.paliurus polysaccharide treatment,respectively.Plasma alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)level decreased 47.47％(P＜0.05),43.65％(P＜0.05)and 50.51％(P＜0.05)in the high-dose group of C.paliurus polysaccharide treatment,respectively.Superoxide dismutase,catalase,glutathione peroxidase,glutathione reductase level increased 1.16 times(P＜0.01),71.28％(P＜0.05),1.29 times(P＜0.01)and 87.46％(P＜0.05)in the high-dose group of C.paliurus polysaccharide treatment,respectively.Enzyme activities of NF-κB,TNF-α,IL-1βand IL-6 were decreased 39.29％(P＜0.05),51.11％(P＜0.05),37.42％(P＜0.05)and 36.50％(P＜0.05),respectively.CONCLUSIONS:Administration of C.paliurus polysaccharide may play a protecting role for liver injury of diabetic rats through lowering glucose,ALT,AST,ALP level,increasing glycogen and insulin level,enhancing the anti-oxidative ability and down-regulating the inflammatory factors expression.
维普
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