首页|Untargeted serum metabonomic reveals alleviated ovalbumin-induced asthma by Baijin Pingchuan(白金平喘)through primary bile acid biosynthesis
Untargeted serum metabonomic reveals alleviated ovalbumin-induced asthma by Baijin Pingchuan(白金平喘)through primary bile acid biosynthesis
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维普
Untargeted serum metabonomic reveals alleviated ovalbumin-induced asthma by Baijin Pingchuan(白金平喘)through primary bile acid biosynthesis
OBJECTIVE:To investigate the effect of baijinpingchuan(白金平喘,BJPC)on the asthma rat model and identify differential metabolites and disturbed metabolic pathways.METHODS:The rats were categorized into six groups:control,dexamethasone(DEX),ovalbumin(OVA),and low-,median-,and high-dose BJPC.The rats,except for the control group,were initially treated with OVA to develop the asthma model,which was then activated using DEX,OVA,and low-,median-,and high-dose BJPC.Enzyme-linked immunosorbent assay kit was used to detect the expression of interleukin(IL)-33,IL-25,thymic stromal lymphopoietin(TSLP),and transforming growth factor-beta 1(TGF-β1).Hematoxylin and eosin staining were performed to observe the pathological condition of the lung.Untargeted serum metabonomic analysis was conducted to identify differential metabolites and disturbed metabolic pathways.RESULTS:High-dose BJPC significantly inhibited the expression of IL-33,IL-25,TSLP,and TGF-β1(P＜0.0001).Further,high-dose BJPC improved inflammatory cell infiltration,which plays a similar role in asthma as DEX.OVA-induced and BJPC-treated rats were identified through 17 differential metabolites,especially cholic acid.Furthermore,primary bile acid biosynthesis was a significantly differential pathway in the mechanism of BJPC for treating asthma.CONCLUSIONS:BJPC plays an anti-inflammation role in asthma,which might be a promising therapy through mediating primary bile acid biosynthesis.
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