基于LRG1/TGF-β信号轴探讨清热活血方调节"炎症-骨破坏"治疗类风湿关节炎的靶向作用机制
Discussion on the Targeting Mechanism of Qingre Huoxue Formula(清活血方)for Treatment of Rheuma-toid Arthritis by Regulating"Inflammation-Bone Destruction":Based on LRG1/TGF-β Signalling Axis
刘蔚翔 1施雨 2张彦琼 3巩勋 1姜泉1
作者信息
- 1. 中国中医科学院广安门医院,北京市西城区北线阁5号,100053
- 2. 中国人民解放军北京卫戍区朝阳第十离职干部休养所
- 3. 中国中医科学院中药研究所
- 折叠
摘要
通过梳理前期相关研究,认为湿热证是类风湿关节炎(RA)的核心证候,清热活血方是治疗RA湿热证的有效方剂."炎症-骨破坏"是RA的关键病理环节,也是清热活血方疗效的优势环节所在,富含亮氨酸α2糖蛋白1(LRG1)可介导转化生长因子-β(TGF-β)信号通路参与RA"炎症-骨破坏"病理过程,或可作为清热活血方治疗RA湿热证的疗效靶点蛋白.据此提出科学假说,即清热活血方可能通过靶向调控LRG1所介导的TGF-β信号通路调节"炎症-骨破坏"治疗RA,并设想后续通过临床研究证实清热活血方对LRG1的临床干预效应,通过动物实验明确清热活血方对LRG1/TGF-β信号轴的作用机制以及LRG1/TGF-β信号轴表达与否对清热活血方的疗效影响.
Abstract
Based on the previous publications,it is believed that damp-heat syndrome is the core syndrome of rheumatoid arthritis(RA),and Qingre Huoxue Formula(清热活血方)is an effective formula for the treatment of damp-heat syndrome of RA."Inflammation-bone destruction"is a key pathological link of RA,and it is also the advantage of the effectiveness of Qingre Huoxue Formula.Leucine rich α-2-glycoprotein 1(LRG1)can mediate the transforming growth factor-β(TGF-β)signalling pathway to participate in the pathogenic process of"inflammation-bone destruction"of RA,and it can be used as a target protein in the treatment of damp-heat syndrome of RA by Qingre Huoxue Formula.Accordingly,a scientific hypothesis was proposed that Qingre Huoxue Formula may regulate TGF-β signalling pathway mediated by LRG1 to improve"inflammation-bone destruction"of RA,and it was envisioned that the clinical effect of Qingre Huoxue Formula on LRG1 could be confirmed through clinical studies,and the mecha-nism of action of Qingre Huoxue Formula on the LRG1/TGF-β signalling axis as well as the influence of the expression or non-expression of the LRG1/TGF-β signalling axis on the therapeutic effectiveness of Qingre Huoxue Formula could be clarified through animal experiments.
关键词
类风湿关节炎/湿热证/清热活血方/炎症/骨破坏/富含亮氨酸α2糖蛋白1/转化生长因子-βKey words
rheumatoid arthritis/damp-heat syndrome/Qingre Huoxue Formula(清热活血方)/inflammation/bone destruction/leucine rich α-2-glycoprotein 1/transforming growth factor-β引用本文复制引用
基金项目
国家自然科学基金(82305224)
国家自然科学基金(82230121)
国家自然科学基金(82274507)
中国中医科学院科技创新工程重大攻关项目(CI2021A01501)
中国中医科学院优秀青年科技人才培养专项(ZZ17-YQ-011)
中华中医药学会风湿病分会青年培英计划人才培养项目(202327-001)
出版年
2024
国家科技期刊平台
NSTL
万方数据