The Effect of Yangyin Xifeng Tongluo Formula(养阴熄风通络方)and Its Core Herb-Pairs on Prevention of Aconitine-Induced Ventricular Precontraction in Rats
Objective To observe the effect of Yangyin Xifeng Tongluo Formula(养阴熄风通络方)and its core herbs combination on prevention of ventricular precontraction(PVC).Methods Experiment 1:Forty SD rats were randomly divided into model group,formula group,core herb-pairs medium-dose group and amiodarone group,with 10 rats in each group.Rats in the model group were given 10 ml/(kg·d)of pure water by gavage,rats in the formula group were given 1.125 g/(kg·d)of Yangyin Xifeng Tongluo Granules(养阴熄风通络方颗粒)by gavage,rats in the core herb-pairs medium-dose group were given 0.585 g/(kg·d)of granules of core herb-pairs by gavage,and rats in the amiodarone group were given 18 mg/(kg·d)of amiodarone hydrochloride tablets by gavage.The rats in each group were gavaged once a day for 14 consecutive days,and then a PVC model was established using rat tail vein injection of aconitine 25 μg/kg to compare the mortality and incidence of PVC,ventricular tachycardia(VT)and ventricular fibrillation(VF),the time of the appearance of PVC,and the duration of PVC in the rats in each group.Experiment 2:Sixty SD rats were randomly divided into model group,the amiodarone group,and the core herb-pairs of low,medium and high dose groups,12 rats in each group.Rats in the model group were gavaged with 10 ml/(kg·d)of purified water,rats in the low,medium,and high dose groups were gavaged with 0.2925,0.585,and 1.17 g/(kg·d)of the core herb-pairs granules respectively,and rats in the amiodarone group were gavaged with 18 mg/(kg·d)of amioda-rone hydrochloride tablets.All of them were gavaged once a day.After 14 days,rats in each group were injected intra-venously into the tail vein with aconitine 25 μg/kg,and then the rats in each group were observed to show the mortality and incidence of PVC,VT and VF,and the time of appearance and duration of PVC.Results Experiment 1:Compared with the model group,The difference in the incidence of PVC in rats of all groups was not statistically signi-ficant(P＞0.05),and the mortality and incidence of VT and VF in rats in the formula group,the core herb-pairs medium-dose group,and the amiodarone group significantly reduced,with delayed time of the occurrence of PVC and shorter duration of the PVC(P＜0.05 or P＜0.01)as compared with the model group,whereas the difference between each group with medication intervention was not statistically significant(P＞0.05).Experiment 2:There was no statistically significant difference in the incidence of PVC in all groups(P＞0.05),and the mortality and inci-dence of VT and VF reduced in the core herb-pairs of low-,medium-,and high-dose groups and in the amiodarone group,and the appearance of PVC delayed and its duration shortened(P＜0.05 or P＜0.01)as compared with the control group,whereas the differences were not statistically significant in the comparison among groups with medica-tion(P＞0.05).Conclusion Both Yangyin Xifeng Tongluo Formula and its core herb-pairs could delay the time of occurrence and shorten the duration of PVC.
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