Effects of Electroacupuncture at"Neiguan"(PC6)-"Zhongwan"(RN12)for Rats with Functional Dyspepsia on Gastric Motility and Metabolites of Antral Tissue
Objective To investigate the possible mechanism of electroacupuncture at"Neiguan"(PC6)and"Zhongwan"(RN12)in the treatment of functional dyspepsia(FD).Methods SPF male SD rats were randomly divided into model group,Neiguan(PC6)group,Zhongwan(RN12)group,and Neiguan-Zhongwan(PC6-RN12)group,with 8 rats in each group.Except for the normal group,the other groups of rats were cogavaged with 0.1％sucrose iodoacetamide solution combined with small platform standing training to establish FD rat models.After suc-cessful modeling,the rats in the normal group and model group were tied up for 30 min/d for 7 days;the Neiguan group,Zhongwan group,and Neiguan-Zhongwan group were treated with electroacupuncture intervention at"Nei-guan"(PC6),"Zhongwan"(RN12),and"Neiguan"-"Zhongwan"(PC6-RN12)acupoints,respectively,using con-tinuous wave,frequency of 2 Hz,current intensity of 1 mA,30 min/d,and continuous intervention for 7 days.The general condition of rats in each group was observed.After treatment,the body weight and food intake of rats were measured,and the gastric emptying rate was calculated;HE staining was performed on the gastric antrum tissue of rats to observe the histopathologic changes;the expressions of calcitonin gene-related peptide(CGRP)and Ghrelin protein in gastric antrum were detected by Western Blot;the metabolites in gastric antrum tissues were detected by liquid chromatography-mass spectrometry(LC-MS),and differential metabolites were screened by correlation analysis,then Metabo Analyst 5.0 and KEGG databases were used for metabolic pathway analysis.Results Under light micro-scope,the gastric antrum structure was complete and the glands were abundant.No obvious inflammation and edema were found in gastric mucosa.Compared with the normal group,the body weight,food intake,and gastric emptie rate of rats in model group decreased,the expression of Ghrelin protein decreased and the expression of CGRP protein in-creased in gastric antrum tissue(P＜0.01).Compared with model group,the body weight,food intake,and gastric emptance rate of rats in Neiguan group,Zhongwan group and Neiguan-Zhongwan group all increased,CGRP protein ex-pression decreased in Neiguan group,and Ghrelin protein expression increased and CGRP protein expression decreased in Zhongwan group and Neiguan-Zhongwan group(P＜0.01 or P＜0.05).Compared with Neiguan-Zhongwan group,Ghrelin protein expression decreased and CGRP protein expression increased in Neiguan group and Zhongwan group(P＜0.05 or P＜0.01).Metabolomics results showed that compared with normal group,the content of metabolites adenosine diphosphate ribose,adenosine monophosphate and adenosine diphosphate in gastric antrum tissue of model group decreased;compared with model group,the contents of adenosine diphosphate,adenosine diphosphate and citico-line in Neiguan group increased,the contents of nicotine adenine dinucleotide,cytidine diphosphate ethanolamine and citicoline in Zhongwan group increased,and the contents of adenosine diphosphate,cytidine diphosphate and citico-line in Neiguan-Zhongwan group increased(P＜0.05 or P＜0.01).Compared with model group,the main metabolic pathways of different metabolites in PC6-RN12 group were glycerophospholipid metabolism,taurine and hypotaurine metabolism,purine metabolism,starch and sucrose metabolism.Conclusion Electroacupuncture at"Neiguan"and"Zhongwan"acupoints can effectively regulate gastrointestinal motility and improve FD symptoms in FD rats,and the effect is better than that of"Neiguan"or"Zhongwan"acupoints alone.The mechanism may be related to the influence of related metabolites on energy metabolism,glucose metabolism and nucleotide metabolism,thereby regulating gas-trointestinal motility hormones.
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