首页|肝郁气滞型失眠实验动物模型复制方法及评价概述

肝郁气滞型失眠实验动物模型复制方法及评价概述

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梳理近年来中医肝郁气滞型失眠病证结合动物模型的研究资料,从实验动物选择、造模方法、模型评价方面进行总结,以期为肝郁气滞型失眠实验模型的规范化制备和干预机制研究提供参考.实验动物选择方面,肝郁气滞型失眠模型中大鼠、小鼠因其睡眠模式与人类较为相似而常用于睡眠模式的研究;斑马鱼因基因同源性高而适用于早期发育中睡眠模式的研究;猕猴、普通狨猴和食蟹猴则因具有与人类更接近的生理和行为特性,被用于高度复杂的睡眠障碍研究.造模方法方面,电刺激法、慢性束缚及慢性情绪应激法各有其特点和应用场景,而慢性夹尾刺激复合腹腔注射对氯苯丙氨酸(PCPA)法结合了物理和化学两种刺激,以模拟人类失眠的复杂机制,能较全面地模拟肝郁气滞型失眠的病理状态.评价指标方面,脑电图(EEG)和肌电图(EMG)监测、巴比妥类药物协同睡眠实验是评价肝郁气滞型失眠动物模型最常用且科学的方法,能够提供直观、连续的睡眠状态监测;巴比妥类药物协同睡眠实验可以反映模型动物对睡眠调节药物的敏感性和响应性;而以方测证评价法的引入将中医的治疗和诊断原则与现代科学研究相结合,为证候模型的评价提供了新思路.
The Replication Method and Evaluation of Experimental Animal Models with Insomnia of Liver Depression and Qi Stagnation Syndrome
In recent years,the research data on the combination model of insomnia of liver depression and qi stagnation syndrome in traditional Chinese medicine(TCM)were sorted out and summarized in terms of the selection of experimental animals,modelling methods,and model evaluation,with a view to providing references for the stan-dardized preparation of the experimental model of insomnia of liver depression and qi stagnation syndrome and the study of the intervention mechanism.As for the selection of experimental animals,rats and mice are commonly used for the study of sleep patterns in the insomnia model of liver depression and qi stagnation syndrome because their sleep patterns are similar to those of human beings;zebrafish is suitable for the study of sleep patterns in early development because of its high genetic homology;and rhesus macaques,common marmosets,and crab-eating monkeys are used for the study of highly complex sleep disorders because their physiological and behavioural characteristics are closer to those of human beings.In terms of modelling methods,electrical stimulation,chronic restraint,chronic emotional stress and impact psychological stress each have their own characteristics and application scenarios,while chronic tail-clamping stimulation combined with intraperitoneal injection of p-chlorophenylalanine(PCPA)combines physical and chemical stimuli,in order to simulate the complex mechanism of insomnia in human beings,and to more comprehen-sively simulate the pathology of insomnia with liver depression and qi stagnation syndrome.In terms of evaluation in-dexes,electroencephalography(EEG)and electromyography(EMG)monitoring and barbiturate synergistic sleep test are the most commonly used and scientific methods for evaluating insomnia animal models with liver depression and qi stagnation syndrome,which can provide intuitive and continuous sleep state monitoring;barbiturate synergistic sleep test can reflect the sensitivity and responsiveness of the model animals to the sleep-regulating medications;and the in-troduction of the method of using formulas to measure syndromes for the evaluating the model can combine the treat-ment and diagnostic principles of TCM with modern scientific research.

insomnialiver depression and qi stagnationcombination of disease and syndromeanimal modelreview

张认真、叶钰娟、魏玉婷、马翠、严兴科

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甘肃中医药大学针灸推拿学院,甘肃省兰州市城关区定西东路35号,730000

失眠 肝郁气滞 病证结合 动物模型 综述

2023年度甘肃省联合科研基金2023年度甘肃省优秀博士生项目2023年度甘肃省省级重点人才项目2023年度研究生创新创业基金2023年度甘肃中医药大学科学研究与创新基金

23JRRA152223JRRA1222甘组通字[2023]20号甘中医大研发[2022]66号2023-19

2024

10.13288/j.11-2166/r.2024.14.013

中医杂志
中华中医药学会 中国中医科学院

中医杂志

CSTPCD北大核心
影响因子：1.464
ISSN：1001-1668
年,卷(期)：2024.65(14)