Effects of Natural Moxibustion at"Feishu(BL 13)"on Th17/Treg Immune Balance and Inflammatory Response in Healthy Rats
Objective To observe the effect of natural moxibustion at"Feishu(BL 13)"on immune balance of T helper cell 17(Th17)/regulatory T cell(Treg)in healthy rats.Methods Forty-eight rats were randomly divided into 10 rats in the sham moxibustion group and 38 rats in natural moxibustion group.The rats in the sham moxibustion group applied blank acupoint stickers to bilateral"Feishu(BL 13)",and the rats in natural moxibustion group applied acupoint stickers filled with Compound Banmao Ointment(复方斑蝥膏)to bilateral"Feishu(BL 13)"fora period of 8 h.Thirty rats in natural moxibustion group were successfully blistered after 8 h,and then were randomly divided into 1-day,3-day and 7-day groups with 10 rats in each group.The general condition of rats was recorded dur-ing the experiment;different time groups of natural moxibustion were sampled at the corresponding time,and HE staining was used to observe the pathological changes of the skin in the area of application;flow cytometry was used to detect the subpopulations of Th17 and Treg in peripheral blood,and the value of Th17/Treg was calculated;and ELISA was used to detect the serum interleukin 17A(IL-17A)and interleukin 6(IL-6),interleukin 10(IL-10)levels.Results Compared with sham moxibustion group,blisters can be seen in the application area of rats in 1-day natural moxibustion group,and the rats often scratched the skin of the moxibustion area,which showed loose stratum corneum,thickening of the stratum spinosum and stratum granulosum,absence of cells in the basal layer,inflammatory cell in-filtration,and elevation of Treg in peripheral blood,and serum IL-17A,IL-6;in 3-day natural moxibustion group,the moxibustion area of the rats was scabbed and partially detached,with the most obvious dermatopathological changes,and elevated peripheral blood Th17 and Treg,and serum IL-17A,IL-6,IL-10;in 7-day natural moxibustion group,skin damage and pathological changes in the area of moxibustion were basically restored;Th17/Treg values were re-duced in 1-,3-and 7-day moxibustion groups after blistering(P＜0.05 or P＜0.01).Compared with the 1-day moxi-bustion group,peripheral blood Th 17,Treg,and serum IL-17A elevated in the 3-day moxibustion group;peripheral blood Treg,serum IL-17A,and IL-6 decreased,and Th17/Treg values elevated in the 7-day moxibustion group(P＜0.05 or P＜0.01).Compared with the 3-day moxibustion group,the 7-day moxibustion group had lower peripheral blood Th 17 and Treg,serum IL-17A,IL-6,andIL-10,and higher Th 17/Treg values(P＜0.05 or P＜0.01).Conclusion Natural moxibustion at"Feishu(BL 13)"can shift the Th17/Treg balance towards Treg of healthy rats,which will gradually lead to immune homeostasis,and regulate the relevant inflammatory factors in the serum to prevent inflammation from occurring and developing.
natural moxibustionFeishu(BL 13)T helper cell 17regulatory T cellimmune balanceinflamma-tory response
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