Bactericidal efficacy and mechanism of Ag/g-C3N4 composites under visible-light-driven
In this paper,the silver loaded graphite phase carbon nitride composites(Ag/g-C3N4)was successfully prepared by calcination-photochemical deposition method.By testing its ability to kill E.coli under visible light,it was found that the optimal composite material could inactivate all the E.coli after 40 minutes irradiation of visible light.The capture experiment indicated that the main active matenal(ROSs)in the photocatalytic sterilization process of the composite materials was superoxide radical(·O2-)and photogenic hole(h+).With the extension of photocatalytic time,it was found that the surface of the bacteria gradually appeared deformations such as depressions,folds and large perforations through scanning electron microscopty and finally all the bacteria were dyed red.In addition,a significant increase of potassium ion(K+)concentration in the reaction system by using a reagent kit,which further confirmed that active substances constantly attacked the cell membrane to destroy its integrity,and then attacked the cytoplasm,disrupting the work of the organelles,changing the internal and external ion exchange,and finally resulting in the leakage of important intracellular substance K+and the inactivation of bacteria.
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