可见光驱动下Ag/g-C3N4复合材料杀菌的效能及机制
Bactericidal efficacy and mechanism of Ag/g-C3N4 composites under visible-light-driven
张欢欢 1侯奉明 2张林林3
作者信息
- 1. 郑州财经学院智能工程学院,河南郑州 450000
- 2. 南京邮电大学信息科学与纳米技术研究院,江苏南京 210023
- 3. 通许县第一高级中学,河南开封 475000
- 折叠
摘要
采用煅烧-光化学沉积法成功制备了银负载石墨相氮化碳复合材料(Ag/g-C3N4),通过考察其在可见光下杀灭大肠杆菌(E.coli)的能力,发现最优复合材料Ag/g-C3N4-3在可见光照射40min后可将E.coli全部灭活.捕获实验表明,复合材料在光催化杀菌过程中的主要活性物质(ROSs)是超氧自由基(·O2-)和光生空穴(h+).随着光催化时间延长,通过扫描电子显微镜(SEM)及共聚焦显微镜发现细菌表面逐渐出现凹陷、褶皱、大穿孔等变形,且菌体最终全部被染成红色.另外,利用试剂盒检测到反应体系中钾离子(K+)浓度显著升高,进一步证实了活性物质不断攻击细胞膜以破坏其完整性,进而攻击细胞质,扰乱细胞器工作、改变内外离子交换,最终造成胞内重要物质K+泄露,细菌失活.
Abstract
In this paper,the silver loaded graphite phase carbon nitride composites(Ag/g-C3N4)was successfully prepared by calcination-photochemical deposition method.By testing its ability to kill E.coli under visible light,it was found that the optimal composite material could inactivate all the E.coli after 40 minutes irradiation of visible light.The capture experiment indicated that the main active matenal(ROSs)in the photocatalytic sterilization process of the composite materials was superoxide radical(·O2-)and photogenic hole(h+).With the extension of photocatalytic time,it was found that the surface of the bacteria gradually appeared deformations such as depressions,folds and large perforations through scanning electron microscopty and finally all the bacteria were dyed red.In addition,a significant increase of potassium ion(K+)concentration in the reaction system by using a reagent kit,which further confirmed that active substances constantly attacked the cell membrane to destroy its integrity,and then attacked the cytoplasm,disrupting the work of the organelles,changing the internal and external ion exchange,and finally resulting in the leakage of important intracellular substance K+and the inactivation of bacteria.
关键词
光催化/大肠杆菌/表面等离子体响应/K+泄漏Key words
photocatalysis/E.coli/the surface plasmon resonance/K+leakage引用本文复制引用
基金项目
河南省教育厅科学规划一般项目(2022YB0395)
郑州财经学院校级青年项目(ZCKY2022-0103)
出版年
2024
NETL
NSTL
万方数据