RESEARCH ON RAPID DETERMINATION OF PROTEIN CONTENT BY SPECTROPHOTOMETRIC METHOD
Based on the spectrophotometry detection technique,we established a rapid analysis method for determining the protein content in samples. In the experiment, the protein in the samples quickly reacted with coomassie brilliant blue G-2S0 to generate a colored combination, the absorption value of the combination at the maximum absorption wavelength of 610 nm was proportional to the protein concentration in the samples,with the linear range of 0 to 100 n-g/mL and linear coefficiency of 0.997. The method completed in 2 minutes,and could be used for the rapid determination of protein content in samples.
spectrophotometryproteincoomassie brilliant blue G-250rapid determination
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维普
