PROGRESS ON THE RESEARCH OF SACCHAROMYCES CEREVISIAE'S BREEDING AND ITS APPLICATIONS
Saccharomyces cerevisiae, which is rich in protein, amino acids, vitamins, fats, edible fiber, trace elements and other physiologically active nutrients, has the functions in nutrition, health and medical care.In addition, Saccharomyces cerevisiae is the major species of alcohol brewing, which is one of the main raw material in food, medicine, chemicals, fuels and other industrial fields.Therefore, Saccharomyces cerevisiae is widely used in brewing, food, medical care, feed, energy chemical industry, industry environmental protection as well as life science research, and is a very important industrial microbial resources.But because of lacking good wine yeast strains in many companies, resulting in high cost of fermentation products with poor quality are produced.So it has great economic value and significance to bred yeast that has many excellent characteristics.The present study was to summary the screening, breeding and identification of the Saccharomyces cerevisiae and its applications.With the development of molecular biology and genetic engineering technology, the breeding method of Saccharomyces cerevisiae is constantly updated, and natural selection, mutation breeding, cross breeding, protoplast fusion, genetic engineering breeding are the commonly used breeding methods.The identification methods of Saccharomyces cerevisiae mainly include traditional identification, molecular identification, DNA homology analysis, pulse electric field gel electrophoresis analysis, DNA sequence analysis, PCR-RFLP and so on.