超声辅助水酶法提取花生油脂工艺研究及油脂品质分析
Study on ultrasonic-assisted aqueous enzymatic extraction process of peanut oil and analysis of oil quality
董艺帆 1贾赛阳 1杨鸣 1陈复生 1段晓杰 1布冠好 1杨趁仙 1朱婷伟1
作者信息
- 1. 河南工业大学 粮油食品学院,河南 郑州 450001
- 折叠
摘要
为了实现花生油脂的高效提取和利用,采用超声辅助水酶法提取花生油脂,研究超声功率、作用时间、作用温度、料液比及酶用量对花生油脂提取率的影响,采用响应面试验优化提取工艺,并分析花生油脂的脂肪酸组成、酸价、过氧化值和抗氧化能力.结果表明:花生油脂的最优提取工艺条件为料液比 1∶5 g/mL、纤维素酶用量 1.0%、超声功率 247 W、作用时间 41 min、作用温度 51℃,此条件下的油脂提取率为 92.64%±0.73%;从提取的花生油脂中共检出 8 种脂肪酸,其中油酸含量最高,为40.44%±0.57%,亚油酸含量为 36.97%±0.05%;花生油脂的酸价为(0.38±0.04)mg/g,过氧化值为(0.15±0.00)g/100 g,DPPH 和 ABTS 自由基清除率分别为 64.82%±2.51%、82.56%±6.47%.超声辅助水酶法有效缩短了提取时间,降低了酶用量,并且制备的花生油脂中不饱和脂肪酸含量高,具有良好的抗氧化能力.
Abstract
To improve the extraction efficiency and utilization of peanut oil,the ultrasonic-assisted aqueous enzymatic method was used to extract peanut oil.The effects of ultrasonic power,treatment time,tempera-ture,solid-liquid ratio,and enzyme dosage on the extraction rate of peanut oil were studied.The extraction conditions were then optimized by response surface methodology.The fatty acid composition,acid value,peroxide value and antioxidant capacity of peanut oil were finally analyzed.The optimal conditions for pea-nut oil extraction were as follows:material to liquid ratio of 1∶5 g/mL,cellulase dosage of 1.0%,ultra-sonic power of 247 W,41 min and 51℃.Under these conditions,the oil extraction rate reached 92.64%±0.73%.A total of 8 fatty acids were detected in peanut oil,among which oleic acid exhibited the highest concentration at 40.44%±0.57%followed by linoleic acid 36.97%±0.05%.The acid value and peroxide value were determined to be(0.38±0.04)mg/g and(0.15±0.00)g/100 g,respectively.Ad-ditionally,the DPPH and ABTS free radical scavenging rates were 64.82%±2.51%and 82.56%±6.47%,respectively.Ultrasonic-assisted aqueous enzymatic method effectively shortened the processing time and reduced the dosage of enzyme.The obtained peanut oil contained high content of unsaturated fatty acids and showed excellent antioxidant capacity.
关键词
花生油脂/水酶法/超声/脂肪酸/抗氧化能力Key words
peanut oil/aqueous enzymatic extraction/ultrasound/fatty acid/antioxidant capacity引用本文复制引用
基金项目
国家自然科学基金项目(U21A20270)
河南省自然科学基金项目(232300420205)
河南工业大学创新基金支持计划专项(2021ZKCJ03)
出版年
2024
NETL
NSTL
万方数据