摘要
柚苷酶是一种具有α-L-鼠李糖苷酶和β-D-葡萄糖苷酶两种酶学活性的酶,在柑橘类产品脱苦、鼠李糖和普鲁宁制备及类固醇的生物转化等方面具有广泛的应用前景.柚苷酶来源广泛,其主要来源为微生物.不同来源柚苷酶的酶学性质往往会存在差异,从而影响其应用领域.系统地从柚苷酶来源、微生物产酶、活力测定、分离纯化与性质、克隆表达、结构、固定化及应用等方面对柚苷酶进行了综述,有助于指导其更好地科学研究和工业化应用.
Abstract
With the ability of α-L-rhamnosidase and β-D-glucosidase,naringinase is an enzyme with a wide range of potential applications,including the biotransformation of steroids,rhamnose synthesis,pru-nasin manufacturing,and the elimination of bitterness from citrus goods.Naringinase is mostly found in microorganisms,while it can also be found in other sources.Naringinases with varying sources frequently display differences in their enzymatic characteristics,which can affect the sectors in which they are used.This paper aims to present a comprehensive overview of naringinase,considering its critical roles.It covers various aspects of the enzyme,including its sources,microbial enzyme production,activity measurement,isolation,purification,characterization,cloning and expression,structure,immobilization,and applica-tions.The goal of this study is to provide a direction for naringinase-related advanced scientific research and industrial applications.
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