Extraction process optimization of anthocyanins from Cercis chinensis flower and their structure characterization
The extract of Cercis chinensis flowers is rich in anthocyanins and possesses good biological ac-tivity.In order to efficiently extract the anthocyanins from Cercis chinensis flowers and fully realize their application value,using flowers as the raw material and ethanol as the extractant an ultrasonic-assisted method was adopted to extract the anthocyanins.The extraction conditions were determined through single-factor experiments and response surface optimization.The results showed that the optimal conditions were a liquid-to-solid ratio of 33∶1 mL/g,an ethanol volume fraction of 77%,an ultrasonic power of 326 W,an ultrasonic time of 15 min,and an ultrasonic temperature of 40℃.Under these conditions,the content of-Cercis chinensis flower anthocyanins was(3.40±0.06)mg/g,which is close to the predicted value.Fou-rier transform infrared spectroscopy was used to analyze the Cercis chinensis flower anthocyanins,indica-ting that the molecular structure of the anthocyanins contains hydroxyl groups,oxygen rings,glycosides,phenyl rings,and methoxy groups.In addition,ultra-high-performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)was used to identify the components of the Cercis chinensis flower antho-cyanins,resulting in the identification of 17 types of anthocyanins and 3 anthocyanin derivatives.The opti-mal extraction conditions for Cercis chinensis flower anthocyanins were obtained through this research,and the composition of the Cercis chinensis flower anthocyanins was studied,providing a theoretical basis for their application in the food industry.
万方数据
维普
