Optimization of extraction,purification process and antioxidation activity of polyphenols from Flos Trollii
In order to improve the high-value utilization of the Flos Trollii polyphenols,the ethanol volume fraction,solid-liquid ratio and extraction time were studied by single-factor and response surface methodolo-gy(RSM)experiments to optimize the ultrasonic-assisted solvent extraction conditions of Flos Trollii poly-phenols.The best macroporous adsorption resin was selected to purify the polyphenols,and the free radical scavenging ability,total antioxidant capacity and reducing power of the polyphenols before and after purifi-cation were determined.The results showed that the optimal extraction conditions were extraction time of 36 min,ethanol volume fraction of 32%,and liquid-solid ratio of 13 mL/g.Under these conditions,the poly-phenols content of Flos Trollii was the highest and reached(40.14±0.24)mg/g.D101 macroporous ad-sorption resin was selected as the best resin for purifying polyphenols.Under the conditions of 1.5 mg/mL of the initial polyphenols concentration,adsorption rate of 1 mL/min,60%ethanol as eluent after adsorp-tion equilibrium,elution flow rate 1 mL/min,the purity of polyphenols increased by 4.88 times from 9.35%±0.12%to 55.04%±0.18%(P<0.05).The purification significantly improved the in vitro poly-phenols of Flos Trollii antioxidant capacity.In summary,ultrasonic-assisted extraction and D101 macro-porous adsorption resin purification not only effectively extract and purify polyphenols from Flos Trollii,but also improve its antioxidant capacity at the same time,which provides a theoretical basis for the develop-ment of Flos Trollii functional foods.
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