Objective To investigate the effect of fibrinogen(Fg)on human neuroblastoma cells(SH-SY5Y)and to elucidate the underlying mechanism.Methods SH-SY5Y cells were treated with graded concen-trations of Fg(0,2,4,and 8 mg/mL)and the effects were assessed using a variety of methods:cell viability was assessed using the Cell Counting Kit-8 Viability Assay;the extent of apoptosis was assessed using the Ho-echst33342/propidium iodide double staining;mitochondrial damage was assessed using COX Ⅳ cell immuno-fluorescence staining and immunoblotting of mitochondrial apoptotic pathway-related markers(Bcl-2,Bax,cy-tochrome c,activated caspase-3).Results Fg treatment resulted in a significant decrease in cell viability,exac-erbated cell apoptosis,and induced mitochondrial dysfunction in SH-SY5Y cells in a concentration-dependent manner.Immunoblotting results indicated that Fg treatment may induce apoptosis by altering the expression levels of mitochondrial apoptotic pathway-related markers.Conclusion Fg caused damage and apoptosis in SH-SY5Y cells via a mechanism that may involve mitochondrial dysfunction.
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