Impacts of LncRNA FGD5-AS1 on the proliferation and apoptosis of oxygen glucose deprivation/reoxygen-ation microglia by regulating the miR-195-5p/TXNIP axis
Impacts of LncRNA FGD5-AS1 on the proliferation and apoptosis of oxygen glucose deprivation/reoxygen-ation microglia by regulating the miR-195-5p/TXNIP axis
Objective To investigate the impacts of long non-coding RNA FGD5-AS1(LncRNA FGD5-AS1)on the proliferation and apoptosis of oxygen glucose deprivation/reoxygenation(OGD/R)microglia by regulating the miR-195-5p/TXNIP axis.Methods Normal microglia B-V2 were used as the control group,and an OGD/R model was established.The model was devided into OGD/R group(without plasmid transfec-tion),si-NC group(FGD5-AS1 negative control transfection),si-FGD5-AS1 group(si-FGD5-AS1 transfec-tion),si-FGD5-AS1+inhibitor-NC group(co transfection of si-FGD5-AS1 and miR-195-5p negative control),and si-FGD5-AS1+miR-195-5p inhibitor group(co transfection of si-FGD5-AS1 and miR-195-5p inhibitor).CCK-8 method was applied to detect the proliferation of cells in each group.Flow cytometry was applied to de-tect the apoptosis rate of cells in each group.QRT-PCR method was applied to detect the mRNA levels of FGD5-AS1,miR-195-5p,and TXNIP in each group.Western blot was applied to detect the expression of TX-NIP protein of cells in each group.Dual luciferase activity was applied to verify the targeting relationship be-tween miR-195-5p and FGD5-AS1,TXNIP.Results Compared with the control group,the survival rate,number of clones formed,and the expression level of miR-195-5p in the OGD/R group decreased(P＜0.05),while the apoptosis rate,the mRNA and protein expression levels of FGD5-AS1,TXNIP in the cells increased(P＜0.05).Compared with the OGD/R group,the si-FGD5-AS1 group showed a decrease in cell apoptosis rate,FGD5-AS1,and TXNIP mRNA and protein expression levels(P＜0.05),and an increase in the cell sur-vival rate,number of clones formed,and miR-195-5p expression level(P＜0.05).In the additional experi-ment,it was found that miR-195-5p inhibitor reversed the ef ects of knocking down FGD5-AS1 on the prolifer-ation and apoptosis of OGD/R cells(P＜0.05).Dual luciferase activity confirmed the existence of targeted binding sites between miR-195-5p,FGD5-AS1,and TXNIP,indicating a targeting relationship(P＜0.05).Conclusion Knocking down LncRNA FGD5-AS1 can affect the proliferation and apoptosis of oxygen glucose deprived/reoxygenated microglia by increasing the expression level of miR-195-5p and decreasing the expres-sion level of TXNIP.
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