Objective To investigate whether treatment with bumetanide could alleviate brain injury af-ter cardiac arrest.Methods A mouse cardiac arrest/cardiopulmonary resuscitation(CA/CPR)model was es-tablished,and 2 mg/kg bumetanide was given intravenously.The control group was treated with the same a-mount of normal saline(vehicle,Veh).The survival rate of mice was monitored 24 hours after operation,and the neurological function and motor function were scored.Histopathological examination was performed on mouse brain sections.HE staining was used to detect brain histopathological changes,Fluoro-Jade B staining was used to evaluate neuronal degeneration,GFAP/Iba-1 staining was used to evaluate brain inflammation.and dihydroethidium staining was used to evaluate brain reactive oxygen species production.Results CA/CPR resulted in decreased survival and significant brain injury in mice,which was characterized by decreased neuro-logical function score,decreased motor function score,increased neuronal death,strong neuroinflammatory re-sponse,and increased production of reactive oxygen species.Compared with the control group,bumetanide could significantly improve survival after CA/CPR and improve the above brain injury manifestations.Conclu-sion Bumetanide could improve survival rate after CA/CPR and alleviate post-cardiac arrest brain injury.
维普
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