丁苯酞通过调控Nrf2通路来改善脑小血管病的认知功能障碍
DL-3-N-butylphthalide can improve cognitive impairment in cerebral small vascular disease by regulating Nrf2 pathway
余静 1史颖颖 2卢祖能 3尹皓4
作者信息
- 1. 430300 武汉市黄陂区人民医院检验科
- 2. 江汉大学医学部
- 3. 武汉大学人民医院神经内科
- 4. 430300 武汉市黄陂区人民医院神经内科
- 折叠
摘要
目的 探索丁苯酞通过调控核因子e2(Nuclear factor erythroid-2,NF-E2)相关的转录因子(Re-lated factor 2,Nrf2)信号通路来改善脑小血管病的认知功能障碍.方法 成功建立脑小血管病(Cerebral small vascular disease,CSVD)SD大鼠模型,将成功建立的脑小血管病大鼠模型随机分为空白组、模型组及治疗组,比较3组大鼠水迷宫实验行为学表现,检测3组大鼠脑组织Nrf2蛋白的水平.结果 模型组大鼠在水迷宫实验中穿越平台次数明显减少(P＜0.05),治疗组能明显改善其穿越平台次数(P＜0.05).Western blot显示模型组Nrf2蛋白的水平明显低于空白组(P＜0.05),而治疗组明显得到改善(P＜0.05).结论 丁苯酞通过调控Nrf2信号通路来改善脑小血管病大鼠模型的认知功能障碍.
Abstract
Objective To explore the effect of butylphthalein on cognitive impairment in cerebral small vascular disease by regulating Nrf2 signaling pathway that is related to nuclear factor erythroid-2(NF-E2).Methods Mouse models of cerebral small vascular disease were established successfully and randomly divided into blank group,model group and treatment group.The behavior data of water maze experiment were com-pared among the three groups.The levels of Nrf2 protein in brain tissue of three groups of mice were detected.Results In the water maze experiment,the times of crossing the platform were significantly reduced in the model group(P＜0.05),and the times of crossing the platform was significantly improved in the treatment group(P＜0.05)Western blot detection of Nrf2 protein changes in the brain tissues of the three groups of mice and indicated that Nrf2 in the model group was significantly lower than that in the blank group(P＜0.05),while Nrf2 in the experimental group was significantly improved(P＜0.05).Conclusion DL-3-N-bu-tylphthalein can improve cognitive impairment in small cerebral vascular disease by regulating Nrf2 signaling pathway.
关键词
丁苯酞/Nrf2/脑小血管病/认知功能障碍Key words
DL-3-N-butylphthalein/Nrf2/Cerebral small vascular disease/Cognitive impairment引用本文复制引用
出版年
2024
NETL
NSTL
万方数据