首页|GENE SILENCING CONSTRUCTS FOR ALTERATION OF SEED FATTY ACID COMPOSITION BY RNAi INDUCED TRANSGENE SILENCING
GENE SILENCING CONSTRUCTS FOR ALTERATION OF SEED FATTY ACID COMPOSITION BY RNAi INDUCED TRANSGENE SILENCING
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NSTL
With the impression to silence the endogenous fad 2-1 gene of Glycine maxL. two gene silencing (Cosuppression, CS and artisense, AS) constructs were designed and produced using vicilin seed specific promoter. In co suppression and antisense, 270 bp region of fad 2-1 was taken which is conserved in FAD2 of Aarabidopsis thaliana which encodes (0-6 desaturate enzyme. Efficacy of these constructs was tested in Arabidopsis thaliana. Confirmation of integration of transgene into host genome was confirmed by PCR using bar gene, specific primer and further by southern hybridization.
Gene Silencingtransgene seed fatty acid
ARCHANA SACHDEV、SUBODH KUMAR SINHA、NIRUPAMA DUBEY
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Division of Biochemistry, Indian Agricultural Research Institute, New Delhi-110012
NSTL
