Y. SUNEETHAK. B. KATHIRIAP. K. KATHIRIAT. SRINIVAS...
11页
查看更多>>摘要：Combining ability analysis of a 10 x 10 diallel excluding reciprocals was undertaken for fruit yield per plant and its component characters in round brinjal. Non-additive gene action was noticed, to be pre-ponderant for fruit yield per plant and the yield component characters studied, A perusal or the gca effects revealed KS 224, PLR I. Morvi 4-2 and jBPR 1 to be good general combiners for fruit yield per plant. These parents had also recorded high per se performance for the trait, indicating their suitability in breeding programmes for development of high yielding hybrids. Among the hybrids. 22 crosses had exhibited significant and desirable sea effects for fruit yield per plant. Of these, nine crosses had recorded desirable sea effects in additionto significantly high per se performance, compared to hybrid mean for the trait. An analysis of these crosses revealed the involvement of a good and a poor general combiner par cent for majority (66, 7%) of the crosses and both good parents (33.3%) in few crosses. The hybrid, KS 224 x PLR 1. involving both good combiner parents for fruit yield per plant, had recorded maximum fruit yield, in addition to desirable sea effects for fruit yield and several other component characters. Hence, it is identifiedas a potential hybrid for commercial exploitation during late summer season.
查看更多>>摘要：Mutagenic effectiveness and efficiency of gamma rays, EMS and combined treatments was studied in terms of M_2 (progenies) lethality and chlorophyll mutations in two cultivars of soybean (Pusa-16 & PK-1042). In general the frequencies of chlorophyll mutations were high in gamma rays and combined treatments. Four types of mutants viz., albina, xantha, chlorine and viridis were observed in the study. Gamma rays were found to be more effective to induce chlorophyll mutations in both the cultivars. PK-1042 cultivar exhibited higher mutagenic efficiency as compared to Pusa-16 in EMS and gamma rays treatment.
查看更多>>摘要：With the impression to silence the endogenous fad 2-1 gene of Glycine maxL. two gene silencing (Cosuppression, CS and artisense, AS) constructs were designed and produced using vicilin seed specific promoter. In co suppression and antisense, 270 bp region of fad 2-1 was taken which is conserved in FAD2 of Aarabidopsis thaliana which encodes (0-6 desaturate enzyme. Efficacy of these constructs was tested in Arabidopsis thaliana. Confirmation of integration of transgene into host genome was confirmed by PCR using bar gene, specific primer and further by southern hybridization.
查看更多>>摘要：Laboratory and field trails were conducted to find out the effect of seed size on seed storage, seed quality and field performance in mustard cv.Pusa Bold. The study revealed that seed size was positively related to germination, seedling dry weight, vigour index, but not consistently to the final seed yield. Seed recovery percent with different size grade was maximum with the sieve size 1.9 to 2.4 mm which constituted 76% of the bulk and rest 24% was mixture of large, small and very-small seed. In the present study germination percentages of seed bulks are often very higher 90-94%. Hence, grading may be beneficial in case of precision sowing and to get uniform field stand or desired plant population under stress conditions.
查看更多>>摘要：Using gene specific primers, a genomic sequence encoding omega-6 desaturase gene (fad2-l) of soybean was PCR amplified, cloned and analyzed for sequence homology. This partial genomic sequence is 191 bp long and untranslated region but has no intronsand 5' untranslated region. This partial gene fragment can be used for screening soybean genomic cDNA libraries for isolation of complete gene.
查看更多>>摘要：The full-length protease inhibitor cDNA was cloned by rapid amplification of cDNA ends (RACE) using RNA extracted from Lens cul inaris im mature seeds and primers designed according'to the conserve regions of legume protease inhibitors. The full-length cDNA was 551 bp and contained a 309 bp open reading frame (ORF) 'encoding a protease inhibitor precursor of 102 amino acids. RT-PCR analysis showed the presence of protease inhibitor mRNA expression in all tested tissues including seed, root, stem, leaf and flower. As the mRNA was detected in all the tested plant tissues, this was considered to be a constitutively expressed gene.
查看更多>>摘要：The pigeonpea cv. P33 was chosen for isolation of promoter through adopter PCR technique. A promoter (PM4) of approx.l kb was isolated and characterized. Sequencing and sequence analysis in silico showed presence of seed specific regulatory motifs. The promoter lacked TAT A box. Transient expression assay with GUS gene using biolistic system of gene delivery in immature seeds of mungbean and cowpea showed the ability ofPM4 promoter to drive the expression of the gene fused to it. The sequence is recognized with Gene bank accession number as A Y 771784.
查看更多>>摘要：Eighty three mungbean germplasm were screened in the field against mungbean yellow mosaic virus. Nine germplasm were found as resistant, 16 as moderately resistant, 11 as moderately susceptible, 37 susceptible and 10 as highly susceptible during 2001.Whereas, 7 germplasm were found as resistant, 14 as moderately resistant, 14 as moderately susceptible, 35 susceptible and 13 as highly susceptible during 2001.
查看更多>>摘要：The term "Protease" includes both "endopeptidases" and "exopep tidases" whereas; the term "proteinase" is used to describe only "endopeptidases" (Ryan, 1990). Majority of Pis studied in plant kingdom originate from three main families namely Leguminosae, Solanaceae and Gramineae. If a food source contains a-supply of digestible protein or carbohydrate and also an inhibitor that acts as a biological check, the larval enzymes cannot digest the food and the larva will not grow and develcp through its normal cycle Pis exhibit a very broad spectrum of activity incluJir.g suppression of pathogenic nematodes like Globodera tabaccum, G. pallid a and Meloidogyne incognita by CpTi (Williamson and Hussey, 1996), inhibition of spore germination and mycelium groWth of Alternaria allernata by buckwheat trypsin/chymotrypsin (Dunaevski et al., 1997) and cysteine protease inhibitors from pearl millet inhibit growth fo many pathogenic fungi including Trichoderma reesei (Joshi et al., 1998). Experiment of Girard et al.(1998) showed the high level of resistance of Pis con felTed by proteolytic cleavage in beetle larvae. Tobacco and peas containing multi-domain PI precursor from Nicotiana alata (Na-PI). showed increased resistance against Helicoverpa armigera (Charityet ah, 1999). There have been many examples of Pis active against certain insect species, both in in-vitro assays against insect gut protease (Pannetier ef al., 1997 ; Koiwa et al., 1998) and in in-vivo artificial diet bioassay (Urwin et al., 1997; Vain et al., 1998).
查看更多>>摘要：A field experiment was conducted during rabi season of 2003-2004 to study the genetic divergence in wheat (Triticum aestivum L). All the genotypes were grouped in 11 clusters. The highest number of genotypes were found in cluster I and II (10 each) followed by cluster III and IV (4 each) and cluster Vand VI (3 each). The maximum and minimum inter cluster distances were observed between cluster IV and cluster X (401.26) and cluster II and cluster IX (94.34), respectively. Highest cluster mean for seedyield per plant was found in cluster IX (22.46 g) and the lowest mean for this character was recorded in cluster VII (12.92), respectively. Leaf area of flag leaj (13.07) was found to contribute maximum towards total divergence, whereas number of grainsspikeld (1.92) recorded the least to contribute total divergence
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