首页|Different suppressive effect of lidocaine on persistent Na~+ current and transient Na~+ current in dorsal root ganglion neurons
Different suppressive effect of lidocaine on persistent Na~+ current and transient Na~+ current in dorsal root ganglion neurons
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
Objective:To investigate the different suppressive effect of lidocaine on persistent Na~+ current and transient Na~+ current in injured or uninjured dorsal root ganglion neurons. Methods: Totally 23 SD rats were randomly divided into 2 groups: control group (n = 10) and chronically compressed DRG (dorsal root ganglion) group (CCD group, n = 13). Rats were anesthetized and DRG was isolated. Single DRG neuron was isolated by enzymatic disassociation method. Persistent Na~+ current (I_(NaP)) and transient Na~+ current (I_(NaT)) were elicited in voltage clamp mode. Results: The presence of I_(NbP) was testified in most DRG neurons (38/46 neurons in CCD group and 31/39 neurons in control group, P ＞ 0. 05). However, the current density of I_(NaP) in CCD group (4. 6 + 0. 6 pA/pF, n = 38 neurons) was greater than that in control group (2. 5 + 0. 4 pA/pF, n = 31 neurons) (P ＜ 0. 05). The characteristics of I_(NaP) was observed and found that I_(NaP) could be blocked by 0. 2 μmol/L tetrodotoxin easily. Furthermore, the does-effect relationship of lidocaine on I_(NaP) and I_(NaT) were also examined. I_(NaP) and I_(NaT) were suppressed by different concentrations of lidocaine , the range for I_(NaP) was 5-20 μmol/L and for I_(NaT) was 0. 05-2 mmol/L. Conclusion: I_(NaP) and I_(NaT) were suppressed by different concentrations of lidocaine. I_(NaP) was suppressed by very low concentration of lidocaine (5-20 μmol/L). However, I_(NaT) could only be blocked by high concentration of lidocaine (0. 05-2 mmol/L).
NETL
NSTL
