查看更多>>摘要：Objective:To investigate the different suppressive effect of lidocaine on persistent Na~+ current and transient Na~+ current in injured or uninjured dorsal root ganglion neurons. Methods: Totally 23 SD rats were randomly divided into 2 groups: control group (n = 10) and chronically compressed DRG (dorsal root ganglion) group (CCD group, n = 13). Rats were anesthetized and DRG was isolated. Single DRG neuron was isolated by enzymatic disassociation method. Persistent Na~+ current (I_(NaP)) and transient Na~+ current (I_(NaT)) were elicited in voltage clamp mode. Results: The presence of I_(NbP) was testified in most DRG neurons (38/46 neurons in CCD group and 31/39 neurons in control group, P ＞ 0. 05). However, the current density of I_(NaP) in CCD group (4. 6 + 0. 6 pA/pF, n = 38 neurons) was greater than that in control group (2. 5 + 0. 4 pA/pF, n = 31 neurons) (P ＜ 0. 05). The characteristics of I_(NaP) was observed and found that I_(NaP) could be blocked by 0. 2 μmol/L tetrodotoxin easily. Furthermore, the does-effect relationship of lidocaine on I_(NaP) and I_(NaT) were also examined. I_(NaP) and I_(NaT) were suppressed by different concentrations of lidocaine , the range for I_(NaP) was 5-20 μmol/L and for I_(NaT) was 0. 05-2 mmol/L. Conclusion: I_(NaP) and I_(NaT) were suppressed by different concentrations of lidocaine. I_(NaP) was suppressed by very low concentration of lidocaine (5-20 μmol/L). However, I_(NaT) could only be blocked by high concentration of lidocaine (0. 05-2 mmol/L).
查看更多>>摘要：Objective: To investigate the distribution of the motilin receptor in the amygdala of rats and its role in regulating the duodenal migrating myoelectric complex (MMC). Methods: The distribution of motilin receptor in the amygdala in adult SD rats was detected by immunohistochemistry methods, and the duodenal interdigestive MMC was recorded via the electrodes implanted in the duodenum and analyzed using a multichannel recorder. Results: Motilin receptor was observed in the amygdala of rats. The great amount of motilin receptor was found in the medial amygdaloid nucleus, which was also abundant in the basolateral nucleus but less abundant in the basomedial amygdaloid nucleus, the central amygdaloid nucleus and the lateral amygdaloid nucleus. The shortening of the duodenal MMC cycle duration and the increase of the amplitude and the frequency of phase Ⅲ were recorded after motilin receptors being bound with exogenous motilin in the amygdala. The effects could be completely blocked by the subdiaphragmatic vagotomy but not by the intravenous injections of atropine, phentolamine or propranolol. Anti-motilin serum could partially block these effects, and the destruction of the basolateral nucleus of the amygdala had no significant effects on the duodenal MMC. Conclusion: Motilin receptor is present in all the subnu clei of the amygdala, with the greatest amount of motilin receptor present in the medial amygdaloid nucleus. Microinjections of motilin in the amygdala can shorten the duodenal MMC cycle duration and increase the amplitude and the frequency of phase Ⅲ. These effects might be accomplished via the amygdala-hy pothalamus-brainstem-vagus pathway, indicating the important role of the amygdala motilin receptor in the duodenal MMC regulation.
查看更多>>摘要：Objective: To explore the expression of DNA polymerase iota in transitional cell carcinoma cells and tissues; Methods: RT-PCR was applied to detect the expression of polymerase iota in BIU87 and T24 cells, then the expression of polymerase iota was also detected in the same way in transitional cell carcinoma which was derived from clinical bladder carcinoma and renal pelvic carcinoma. Results: The expression of Pol was low in bladder normal membrana mucosa but significantly elevated in transitional cell carcinoma cells. Compared with the expression of polymerase iota in bladder normal mucous membranes, the expression of polymerase iota was significantly increased in transitional cell carcinoma tissue (P ＜ 0. 01) and associated with the grade of transitional cell carcinoma. Conclusion: The significantly increased expression of polymerase iota may be associated with the generation and development of transitional cell carcinoma, even with its high heterogenicity.
查看更多>>摘要：Objective: Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are X-linked disorders caused by mutations in the dystrophin gene. The majority of recognized mutations are copy number changes of individual exons. The objective of the present study was to assess the multiplex ligation-dependent probe amplification (MLPA) effects of detection of gene mutations. Methods: Samples of 20 control males and 80 males and their mothers referred to our diagnostic facility on the clinical suspicion of DMD or BMD were tested by MLPA and multiplex PCR. Results: The mean DQs for all peak of 20 control male samples was 1. 02 (range from 0. 83 to 1. 21) by MLPA. Deletions or duplications were identified in 6 out of 31 families that had been previously tested as negative by multiplex PCR. One case of complex rearrangement involving a duplication of two regions: dupEX3-9 and dupEX 17-41 were found by MLPA. Conclusions: MLPA is a highly sensitive method and rapid alternative to multiplex PCR for detection of DMD and BMD.
查看更多>>摘要：Objective: To optimize the ELISA for the determination of tetrodotoxin. Methods: A competitive enzyme-linked immunosorbent assay (ELISA) was used. In the ELISA, 100 μl antigen (1.0 μg/ml) was coated on the microtiter plate for 60 min at 37℃ or over night at 4℃. The plate was then washed 3 times with PBS-T for 3-5 s each time. The optimal incubation time for monoclonal antibody (mAb), goat anti-mice IgG peroxidase conjugate and OPD were 30 min, 20 min and 10 min at 37 C , respectively. Results: The detection limit is 0. 05 ng in each well. The curve was linear for TTX doses between 5-5 000 ng/ml (0. 25-250 ng for every assay). The linear regress equation was Y = 0. 30 88X -0. 17 41 (R = 0. 99 01). The average callback for TTX of muscles and gonads were 99. 74% and 100. 30% , respectively. The sensitivity of optimization ELISA was 5 times than traditional method and the time of 1.8 h were saved. Conclusion: The optimized ELISA is an ideal method for the determination of tetrodotoxin.
查看更多>>摘要：Objective: To investigate serum levels of MMP-2 ,MMP-9, oxidized low-density lipoprotein (ox-LDL) in Alzheimer's disease (AD) patients and study the possible pathway and mechanism of AD with abnormal lipid metabolism. Methods: Subjects in this study were divided into 4 groups: normal lipid group without AD (N), hyperlipoidemia group without AD (H), normal group with AD (A), hyperlipoidemia group with AD (AH). There were 15 individuals in each group. MMP-2, MMP-9, ox-LDL was measured by enzyme linked immunosorbent assay (ELISA). Serum lipids levels were measured by biochemical methods. Results: The serum levels of MMP-2, MMP-9, ox-LDL were significantly higher in H, A and AH groups than those in N group. Those of ox-LDL in H, AH groups was higher than that of in A group. The serum level of MMP-2, MMP-9 in AH groups were higher than that of in H group. The score of mini-mental state examination (MMSE) in A and AD groups was negatively correlated with the serum level of ox-LDL. Relationship between the score of MMSE and the serum level of ox-LDL in AD groups and non-AD groups had statistical significance. Conclusion: MMP-2, MMP-9, ox-LDL and abnormal lipid metabolism may participate in pathogenesis of AD, in which abnormal lipid metabolism induces expressions of MMP-2,MMP-9 and ox-LDL. Oxidative stress and blood-brain barrier disruption might accelerate the process of AD.
查看更多>>摘要：Objective: To evaluate the efficacy and safety of the treatment of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Methods: The randomized controlled trials (RCTs) about the treatment for CP/CPPS all over the world were searched. MEDLINE (January 1966 to June 2007), EMBASE (January 1988 to June 2007), and 4 Chinese databases were electronically searched. The studies included in the references of eligible studies were additionally searched. Two reviewers independently screened the studies for eligibility, evaluated the quality and extracted the data from the eligible studies, with confirmation by crosschecking. Divergences of opinion were settled by discussion or consulted by the experts. Meta-analysis was performed by using RevMan 4. 2 software. Results: Twelve original studies involving 1 003 participants met inclusion criteria. Compared with placebo, alpha-blockers could improve the symptoms of CP/CPPS obviously with WMD of NIH-CPSI, total score and pain score were -4. 10 (95%CI: -6. 92 to -1. 28) and -1. 68 (95%CI: -2. 54 to -0. 82). Antibiotics could not improve the symptoms obviously with WMD of NIH-CPSI; total score and pain score were -2. 71 (95%CI: -4. 78 to -0. 64) and -0. 86 (95%CI: -2. 07 to 0.36). Flavoxate could not improve the NIH-CPSI total score obviously, but could relieve the pain, with WMD of NIH-CPSI total score and pain score being -2. 96 (95%CI: -5. 17 to -0. 74) and -2. 31 (95% CI: -4. 05 to 0. 03). Prostat could improve the NIH-CPSI total score obviously, but could not relieve the pain, with WMD of NIH:CPSI total score and pain score being -7. 60 (95%CI: -9. 97 to -5. 23) and - 2. 02 (95%CI: -4. 07 to 0. 04). Conclusion: Drug intervention could improve total symptoms of CP/CPPS in some degree, but no universally effective treatment is available that can prove significant lasting benefit for all the symptoms of CP/CPPS. Future RCT must use an appropriate sample size and optimal duration and follow-up of participants. It is important to improve the quality of internal original studies.
查看更多>>摘要：Objective: Although evidence has already demonstrated that the performance of Health Delivery System (HDS) varies widely across nations, relatively little is known about the factors that give rise to these variations and the key point to improve the performance besides adjusting system structure. By setup of HDS performance measurement system on the base of association of financial, social, and environmental characteristics, we construct system dynamic model of HDS to simulate the invention policies. Methods: Performance measures were collected from HDS in 31 regions of China and combined with secondary data sources. Multivariate, linear, nonlinear regression and factor analysis models were used to estimate associations between system characteristics and the performance. Results: Performance varied significantly with the size, financial resources and organizational structure of HDS. Performance measurement system of health delivery system was developed to give the rank of all Chinese regions. Conclusion: Performance measurement system of HDS is the basic of HDS modeling by system dynamic.
查看更多>>摘要：Objective: To address HBV serum nucleic acid markers for stages without apparent replication. Methods: DNA and RNA sequence segments from the X, C and pre C/C regions produced successively during replication were used as targets for quantitative PCR and RT/PCR. Results: The assays confirmed the preferential formation of intermediates blocked at early stages. They persisted as the only detectable type of serum HBV DNA even after one year of therapy. At reentry into viral replication due to e-mergence of drug resistant mutants, lamivudine resistance produced exclusively incomplete DNA minus strands, whereas the wild type virus immediately synthesized complete DNA minus strands. Conclusion: PCR assays used for monitoring complete suppression of HBV replication must target the X gene region.
查看更多>>摘要：To identify gene expression profiling in epithelial ovarian cancer and to explore its correlation with histopathology characterization and prognosis. Gene expression profiles were generated from 10 human ovarian frozen tissue specimens using Agilent Human 1A microarrays. Strikingly, clear differences of gene expression patterns were observed in ovarian cancer as compared to normal tissues. Unique gene profiles were observed in moderately and poorly differentiated epithelial ovarian cancer. It is concluded that different histopathology characterization likely exists extensive molecular heterogeneity.
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