Abstract
In the study, the protective activity of the total flavonoids (TFs) from Rosa laevigate Michx fruit was evaluated in paracetamol-induced liver damage in mice. TFs (50, 200 mg/kg) were administered via gavage daily for 5 days before paracetamol (400 mg/kg, i.v.) treatment. After the experiment, the levels of serum ALT (alanine aminotransferase), AST (aspartate aminotransferase) and MDA (malondialdehyde) were decreased by 78.42%, 70.85% and 30.52%, respectively, while SOD (superoxide dismutase) and GSH (reduced glutathione) were significantly increased compared with model group (p < 0.05). Further mechanisms study indicated that the TFs could significantly increase the protein expressions of Procaspase-3, Procaspase-8, FasL, Prohibitin and Bcl-2, and markedly decrease the protein expressions of Fas, Bax, p53 and NF-kB p65 compared with the mice treated by paracetamol. Meanwhile, the mRNA level of TNF-α was also down-regulated after the TFs treatment. Our results suggest that the TFs with a striking beneficial activity can be developed to be a potential hepatoprotective drug.
NETL
NSTL
Elsevier