Saccharum Granorum ameliorated mitochondrial dysfunction in chronic atrophic gastritis rats using organelle-specific metabolomics and network pharmacology approaches
颗粒糖改善慢性萎缩性胃炎大鼠线粒体功能障碍的器官特异性代谢组学和网络药理学研究
Liu, Yuetao 1Li, Xinqi 1Qin, Xuemei1
作者信息
- 1. Shanxi Univ, Modern Res Ctr Tradit Chinese Med, 92 Wucheng Rd, Taiyuan 030006, Shanxi, Peoples R China
- 折叠
摘要
粮糖(YiTang)是一种营养丰富的食品，对慢性萎缩性胃炎(CAG)有显著疗效然而，其机制仍不清楚。本文采用超高效液相色谱-四极杆质谱联用技术(UHPLC-Q Exactive MS)研究了其对CAG所致能量障碍的保护作用。首次筛选出16种内源性代谢产物来表征CAG相关线粒体功能障碍。益汤预处理可显著调节这些靶向代谢产物参与脂肪酸β氧化途径和氨基酸代谢。网络药理学进一步表明这些靶向代谢产物的上游蛋白与益汤成分具有良好的亲和力。这些发现为益汤在CAG大鼠体内的局部代谢调节提供了独特的视角，为CAG的发病机制及临床诊断和治疗提供了新的思路。
Abstract
Saccharum Granorum (YiTang) is a nutrient-rich food which could significantly treat chronic atrophic gastritis (CAG). However, its mechanism remains unclear. In the present work, a mitochondria-specific metabolomics approach based on ultra high performance liquid chromatography coupled with quadrupole - Exactive mass spectrometry (UHPLC-Q exactive MS) was carried out to evaluate its protection against energy dysfunction induced by CAG. Sixteen endogenous metabolites were firstly screened out to characterize the mitochondria dysfunction associated with CAG. Pretreatment of YiTang could significantly regulate these targeted metabolites involved into fatty acid beta-oxidation pathway and amino acid metabolism. Network pharmacology further showed the upstream proteins related to these targeted metabolites exerted good affinities with ingredients of YiTang. These findings provided a unique perspective on localized metabolic regulation of YiTang in rats with CAG, which gave us new insights into the pathogenesis of CAG, and its targets for clinical diagnosis and treatment.
Key words
Saccharum Granorum/Chronic atrophic gastritis/Mitochondrial dysfunctions/Metabolomics/Network pharmacology引用本文复制引用
出版年
2020
NETL
NSTL
Elsevier