首页|Circulating tRNA-derived fragments are decreased in patients with rheumatoid arthritis and increased in patients with psoriatic arthritis

Circulating tRNA-derived fragments are decreased in patients with rheumatoid arthritis and increased in patients with psoriatic arthritis

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Introduction: tRNA-derived fragments (tRFs) play an important role in immune responses. To clarify the role of tRFs in autoimmunity we studied circulating tRF-levels in patients with rheumatoid arthritis (RA) and psoriatic arthritis (PsA), and in a murine model for arthritis. Material and methods: Circulating tRF-levels were quantified by miR-Q RT-qPCR. tRNA processing and modification enzyme expression was analysed by RT-qPCR and public transcriptomics data. Results: Significant reduction (up to 3-fold on average) of tRF-levels derived from tRNA-Gly-GCC,CCC, tRNA-Glu-CTC and tRNA-Val-CAC,AAC was observed in RA patients, whereas tRNA-Glu-CTC and tRNA-Val- CAC,AAC tRFs were found at significantly higher levels (up to 3-fold on average) in PsA patients, compared to healthy controls. Also in arthritic ILIRa-KO mice reduced levels of tRNA-Glu-CTC fragments were seen. The expression of NSUN2, a methyltransferase catalysing tRNA methylation, was increased in RA-peripheral blood mononuclear cells (PBMCs) compared to PsA, but this is not consistently supported by public transcriptomics data. Discussion: The observed changes of specific tRF-levels may be involved in the immune responses in RA and PsA and may be applicable as new biomarkers. Conclusion: Circulating tRF-levels are decreased in RA and increased in PsA and this may, at least in part, be mediated by methylation changes.

Rheumatoid arthritispsoriatic arthritiscirculating tRNA fragmentsIL1Ra knockout micetRNA methylation

Marina Dunaeva、Jan Blom、Rogier Thurlings、Margot van Weijsten、Fons A.J. van de Loo、Ger J. M. Pruijn

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Department of Biomolecular Chemistry, Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands

Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands

Department of Rheumatology, Radboud University Medical Center, Nijmegen, The Netherlands

Department of Synthetic Organic Chemistry, Radboud University, Nijmegen, The Netherlands

Department of Rheumatology, Experimental Rheumatology, Radboud University Medical Center, Nijmegen, The Netherlands

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2024

10.1080/1354750X.2024.2319297

Biomarkers

Biomarkers

SCI
ISSN：1354-750X
年,卷(期)：2024.29(1/8)
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