首页|Investigation of exosomal tetraspanin profile in sepsis patients as a promising diagnostic biomarker
Investigation of exosomal tetraspanin profile in sepsis patients as a promising diagnostic biomarker
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NETL
NSTL
Taylor & Francis
Introduction: Sepsis, a leading cause of mortality globally, has a complex and multifaceted pathophysiology which still requires elucidation. Therefore, this study aimed to analyze and quantify the number of exosomes in sepsis patients from a South African cohort using the ExoView (NanoView Biosciences, Boston, MA) platform. Methods: Blood samples were collected from black South African patients attending the local Intensive Care Unit (ICU) hospital. Exosomes were isolated and characterize via TEM and CD63 ELISA kits. ExoView was used to determine particle count, particle size distribution and colocalization of different tetraspanin markers. Results: Exosomal levels in sepsis patients were significantly higher compared to the control group (p<0.05). Sepsis exosomes showed a homogenous size distribution ranging from 55 to 70nm. Tetraspanin colocalization analysis revealed that sepsis exosomes have significantly higher CD63/CD9, CD63/CD81 and CD63/CD9/CD81 colocalization percentages than the control group. Conclusion: This unique tetraspanin colocalization pattern of sepsis exosomes could serve as a potential sepsis biomarker. Further investigations are required to identify sepsis exosomal cargo signatures for further understanding of sepsis pathophysiology in order to develop effective diagnostics and treatments.
Roushka Bhagwan Valjee、Irene Mackraj、Roshila Moodley、Usri H. Ibrahim
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Discipline of Human Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa
Department of Chemistry, The University of Manchester, Manchester, UK
NETL
NSTL
Taylor & Francis
