首页|Methionine supplementation improves freezability of Jamnapari Buck semen

Methionine supplementation improves freezability of Jamnapari Buck semen

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  • NETL
  • NSTL
  • Triveni Enterprises
Aim: The study aimed to assess the effect of methionine supplementation as a semen additive to augment the freezability of Jamnapari buck semen. Methodology: Atotal of forty-eight ejaculates were collected from four Jamnapari bucks (n=12×4) twice a week using an artificial vagina from October to November 2023. The sperm concentration was estimated using a photometer and semen was diluted with tris-citrate-fructose-yolk extender and divided into four equal aliquots (control, T1, T2 and T3) and methionine was added @ 0,2,2.5, and 5.0 mM in C., T1, T2 and T3, respectively. The post-diluted and frozen-thawed semen was evaluated for sperm motility, viability, acrosomal integrity, sperm abnormalities, sperm plasma membrane integrity, sperm penetration distance, and enzyme leakage (AST, ALT, ACP and AKP). Results: Methionine applied @ 2.0 and 5.0 mM in the extender seemed to be less effective at maintaining sperm structural integrity and functionality in comparison to the 2.5 mM treatment. The inclusion of 2.5 mM methionine has significantly enhanced the freezability of Jamnapari buck semen. Interpretation: In conclusion, methionine in tris-citrate-fructose-yolk extender can improve the sperm quality and fertility potential of post-thawed spermatozoa of Jamnapari buck.

CryopreservationJamnapari bucksMethionineSemen

K. Alam、S. Srivastava、S.K. Verma、A. K. Verma、B. Singh、Saurabh、R. Kumar、A. Jaiswal、R.K. Verma、A. Alam、J. Singh、R.R. Singh、P. Kumar、H.C. Verma、N. Singh

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Department of Veterinary Gynaecology & Obstetrics, AcharyaNarendra Deva University of Agriculture and Technology, Kumarganj-224 229, India

Department of Veterinary Clinical Complex, AcharyaNarendra Deva University of Agriculture and Technology, Kumarganj-224 229, India

Department of Livestock Production Management, AcharyaNarendra Deva University of Agriculture and Technology, Kumarganj-224 229, India

Department of Veterinary Medicine, AcharyaNarendra Deva University of Agriculture and Technology, Kumarganj-224 229, India

Department of Animal Genetics & Breeding, AcharyaNarendra Deva University of Agriculture and Technology, Kumarganj-224 229, India

Department of Soil Science, College of Agriculture, AcharyaNarendra Deva University of Agriculture and Technology, Kumarganj-224 229, India

Department of Veterinary Physiology & Biochemistry, AcharyaNarendra Deva University of Agriculture and Technology, Kumarganj-224 229, India

Department of Veterinary and Animal Husbandry Extension Education, College of Veterinary Science & Animal Husbandry, AcharyaNarendra Deva University of Agriculture and Technology, Kumarganj-224 229, India

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2025

10.22438/jeb/46/3/MRN-5402

Journal of environmental biology

Journal of environmental biology

ISSN：0254-8704
年,卷(期)：2025.46(3)
  • 44