Abstract
Introduction: Rapid spread of antimicrobial drug resistance is alarming and demands a sustainable solution. Green synthesis of silver nanoparticles can pave the way for the development of adjuvants that can help overcome bacterial drug resistance. Methods: In the current study, green synthesis of silver nanoparticles utilizing pea (Pisum sativum) peels and their evaluation against multidrug-resistant (MDR) bacterial pathogens has been - proposed. Pea peels were extracted and analyzed for antioxidant potential by non-enzymatic DPPH assay. The extract was used to prepare silver nanoparticles (gAgNPs) characterized using - UV-VIS spectroscopy, SEM, FTIR, and zeta sizer, and evaluated for antibacterial activity. Results: The SEM showed that the gAgNPs were spherical with a size of 59.24 nm ± 0.3, and the zeta sizer gave a PDI of 0.2. As adjuvants, these gAgNPs in combination with vancomycin showed a superior antibacterial activity against vancomycin-resistant E. faecalis (zone of inhibi-tion=66 mm ± 0.41) and vancomycin-resistant P. aeruginosa (35 mm ± 1.41) as compared to vancomycin. Hemolysis was IC_(50) =998.44 μg/mL, creating a therapeutic window for IC_(50) =5 μg/mL, effective against human pathogens. Conclusion: This study suggests these gAgNPs could be a good lead to work as adjuvants with vancomycin against vancomycin-resistant Gram-positive and Gram-negative clinical bacteria and thus warrants further studies.
NETL
NSTL
Bentham Science Publ Ltd