首页期刊导航|Current pharmaceutical biotechnology
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Current pharmaceutical biotechnology
Bentham Science Publishers Ltd.
Current pharmaceutical biotechnology

Bentham Science Publishers Ltd.

双月刊

1389-2010

Current pharmaceutical biotechnology/Journal Current pharmaceutical biotechnologySCIISTP
正式出版
收录年代

    Virus-like Particles-based Vaccine to Combat Neurodegenerative Diseases

    Luis Wei-Cheng LimJia-Yee LeeChing-Hsein ChenKenny Gah-Leong Voon...
    2631-2639页
    查看更多>>摘要：Neurodegenerative diseases are regarded as gradual, incurable conditions with an insidious onset. Alzheimer's disease (AD) and Parkinson's disease (PD) are two of the most prevalent neurodegenerative diseases reported globally. Developing effective treatment strategies for neurodegenerative diseases has remained a primary objective and a huge challenge for researchers. The therapeutic medications that are now approved for the treatment of neurodegenerative diseases merely treat the symptoms; the underlying pathology is not addressed. Therefore, the emergence of novel disease-modifying therapeutic modalities such as immuno-therapy has opened a new path in developing effective treatments for neurogenerative diseases. Compared to other types of subunit active vaccines, virus-like particles (VLPs) are considerably more immunogenic as they present dense and repetitive viral antigen epitopes on their surface, which can trigger both humoral and cell-mediated immune responses. They are also a much safer option than the traditional inactivated and live-attenuated vaccines since they are devoid of viral genomes and are, therefore, non-pathogenic and non-infectious. Researchers have turned their attention to VLPs as an active immunotherapy candidate for AD due to the lessons learned from the AN 1792 trial. Studies have shown that they effectively induce anti-Ap, anti-tau, and anti-a-synuclein antibodies while avoiding T-cell-related immune reactions in animal models of AD and PD. This review compiles the findings of preclinical animal model studies and clinical investigations on VLP-based vaccines for neurogenerative diseases thus far. The technical limitations and potential difficulties associated with the future application of VLP-based vaccines in patients with neurodegenerative diseases have also been covered.
      原文链接:
    • NETL
    • NSTL
    • Bentham Science Publ Ltd

    Tryptophan Stability and Palatability in the Food Formulation: A Review

    Sevda AllahmadadiAziz Homayouni-RadMahnaz TabibiazarPouria Gharehbeglou...
    2640-2648页
    查看更多>>摘要：Tryptophan, an essential amino acid, plays a vital role in the synthesis of critical compounds like serotonin, melatonin, and niacin, which impact mood, sleep, and metabolic processes. It holds promise for improving the well-being of individuals with mood issues or sleep disorders through dietary enrichment. However, incorporating tryptophan into food products presents challenges related to stability, bitterness, and susceptibility to oxidative degradation. These issues can reduce consumer acceptability and effectiveness and may lead to the formation of harmful byproducts. This review comprehensively examines innovative strategies for enriching food products with tryptophan. Crucial approaches include using nano-emulsion systems to encapsulate tryptophan, thereby protecting it from environmental factors and enhancing its bioa-vailability, adding antioxidants to prevent degradation, and utilizing functional derivatives as alternatives to pure tryptophan. These strategies aim to improve the stability of tryptophan, reduce bitterness, and enhance consumer acceptability. This review provides valuable insights into practical methods for incorporating tryptophan into food formulations, with the goal of optimizing its health benefits and ensuring a positive consumer experience.
      原文链接:
    • NETL
    • NSTL
    • Bentham Science Publ Ltd

    Considerations and Challenges to Develop Drug-drug Coamorphous System: A Recent Update

    Madhura TiwariKavita SinghBappaditya Chatterjee
    2649-2663页
    查看更多>>摘要：Poor water solubility of several drugs, especially BCS class II and IV drugs, restricts their dissolution and negatively affects oral absorption. Amorphization of drugs is a year-old approach to enhance solubility and dissolution of poorly water-soluble drugs. Polymeric amorphous systems have been proven effective but have disadvantages, such as low drug loading, high carrier content, etc. In a coamorphous system, a small molecule can be used as a coformer that keeps the amorphous form of a drug stable. In a drug-drug coamorphous system (CAS), one therapeutically active moiety can act as a coformer for the other drug. Although effective, the rationale of selecting the drugs and optimising the ratio without compromising therapeutic effect and safety is challenging. The preparation method is also a challenge because the stress during the processing method may result in the loss of crystallinity. Hence, the processing stability of the amorphous drug is a significant concern. A stable CAS is formed when two drugs generate some molecular-level interaction. In silico prediction of miscibility, molecular dynamic simulation, functional group analysis by Fourier Transform infrared spectroscopy, Raman spectrosco-py, NMR, etc. contribute to the analysis of molecular-level interaction. Additionally, the article discusses the preparation method and the selection of excipient to form an effective CAS.
      原文链接:
    • NETL
    • NSTL
    • Bentham Science Publ Ltd

    Influence of Nanomedicine as a Smart Weapon for Multidrug Resistance in Cancer Therapy

    Safal Kumar PaikrayLiza SahooNigam Sekhar TripathyFahima Dilnawaz...
    2664-2683页
    查看更多>>摘要：Cancer is the leading cause of death worldwide. The effectiveness of chemotherapy in cancer patients is still significantly hampered by multidrug resistance (MDR). Tumors exploit the MDR pathways to invade the host and limit the efficacy of chemotherapeutic drugs that are delivered as single drugs or combinations. Further, overexpression of ATP-binding Cassette transporter (ABC transporter) proteins augments the efflux of chemotherapeutic drugs and lowers their intracellular accumulation. Recent progress in the development of nanotechnology and nanocarrier-based drug delivery systems has shown a better perspective with respect to the improvement of cancer chemotherapy. Nanoparticles/nanomaterials are designed to target the immune system and tumor microenvironment of cancer cells for a variety of cancer treatments in order to improve bioavailability and reduce toxicity. This review elucidates the successful use of nanomaterials for cancer therapy and addressing the MDR and throws some light on the present obstacles impeding their translation to clinical use.
      原文链接:
    • NETL
    • NSTL
    • Bentham Science Publ Ltd

    The Exosome and its Application in Skin Wound Healing: A Systematic Review on In vitro Studies

    Mohammad Bagher HeydariZahra Ghanbari-MovahedMaryam HeydariMohammad Hosein Farzaei...
    2684-2696页
    查看更多>>摘要：Background: Wound healing is a complex procedure frequently delayed in patients with underlying chronic conditions. Despite essential advances in tissue engineering and regenerative medicine, wound healing remains challenging, warranting novel methods for promoting wound healing. It has been demonstrated that exosomes are one of the main secretory products of different cell types, such as Mesenchymal Stem Cells (MSCs), for regulating various biological processes, including wound healing. Henceforth, understanding these exosome effects might assist in improving wound management and highlight a novel therapeutic model for cell-free therapies with reduced side effects for repairing wounds. Methods: This systematic review involved conducting research electronically on scholarly scientific databases, including PubMed, Science Direct, and Scopus. Eligibility checks were performed based on predefined selection criteria. Finally, thirty-nine studies were considered. Results: Exosomes have been indicated to use multitargeted pathways to improve wound healing by modulating numerous dysregulated signaling cascades involved in cell proliferation, cell cycle regulation, metastasis, apoptosis, and angiogenesis. Conclusion: The outcome of this review might guide pre-clinical and clinical studies on the role of exosomes in skin wound healing.
      原文链接:
    • NETL
    • NSTL
    • Bentham Science Publ Ltd

    COL1A1, COL1A2, CHN1, and FN1 Promote Tumorogenesis and Act as Markers of Diagnosis and Survival in Gastric Cancer Patients

    Yichao YanYongbai LiHailiang LiangDongbo Chen...
    2697-2710页
    查看更多>>摘要：Objectives: This study aimed to comprehensively investigate the molecular landscape of gastric cancer (GC) by integrating various bioinformatics tools and experimental validations. Methods: GSE79973 dataset, limma package, STRING, UALCAN, GEPIA, OncoDB, cBi-oPortal, DAVID, TISIDB, Gene Set Cancer Analysis (GSCA), tissue samples, RT-qPCR, and cell proliferation assay were employed in this study. Results: Analysis of the GSE79973 dataset identified 300 differentially expressed genes (DEGs), from which COL1A1, COL1A2, CHN1, and FN1 emerged as pivotal hub genes using protein-protein interaction network analysis. Subsequent validation across The Cancer Genome Atlas (TCGA) datasets confirmed their up-regulation in GC tissues compared to normal controls. Promoter methylation analysis revealed decreased methylation levels of these hubs in GC tissues, suggesting their potential role in tumorigenesis. Mutational analysis using cBioPortal showcased frequent mutations in these genes, particularly FN1, further highlighting their significance in GC pathogenesis. Survival analysis indicated their correlation with reduced overall survival rates among GC patients, supported by the development of a robust prognostic model. Prediction of hub-associated miRNAs and gene enrichment analysis provided insights into their regulatory mechanisms and downstream pathways, implicating their involvement in extracellular matrix remodeling and cell migration. Drug sensitivity analysis revealed correlations between hub gene expression and drug response, while RT-qPCR validation confirmed their up-regulation in clinical GC samples. Finally, functional assays demonstrated the impact of FN1 knockdown on cellular proliferation, colony formation, and wound healing capacities. Conclusion: Overall, this study elucidates the crucial role of COL1A1, COL1A2, CHN1, and FN1 in GC pathogenesis and underscores their potential as diagnostic markers and therapeutic targets.
      原文链接:
    • NETL
    • NSTL
    • Bentham Science Publ Ltd

    In vitro Investigation of Antimicrobial and Antioxidant Properties of Green Silver Nanoparticles Synthesized Using Ephedra gerardiana Plant Extract

    Ali Abizi-MoqadamSobhan Mortazavi-DerazkolaMajid Zare-BidakiFreshteh Osmani...
    2711-2723页
    查看更多>>摘要：Background: The increasing prevalence of antibiotic-resistant bacteria necessitates exploring nanotechnology as a potential solution for microbial elimination. Objectives'. This study aimed to investigate the antimicrobial and antioxidant effects of silver nanoparticles synthesized using aqueous extract from the Ephedra gerardiana (E. gerardiana) plant (EG@AgNPs). Methods: Optimal synthesis conditions, including silver nitrate concentration, time, and temperature, were determined. Characterization of EG@AgNPs was conducted, which was followed by antimicrobial assessment against eight bacterial strains and one fungal strain. Additionally, the antioxidant properties of EG@AgNPs were evaluated using the DPPH method. Results: XRD analysis confirmed EG@AgNPs synthesis. DLS analysis revealed a hydrodynam-ic diameter of 22 nm. FT-IR analysis confirmed the presence of functional groups from the E. gerardiana plant extract in EG@AgNPs. FESEM and TEM images depicted spherical nanoparticles ranging in size from 10 to 20 nm. Antimicrobial investigations using the broth microdilu-tion method demonstrated that E. gerardiana plant extract at 7.5 μg/ml inhibited only Streptococcus mutans and Candida albicans growth, with no antimicrobial effects observed at lower concentrations. However, EG@AgNPs significantly enhanced the antimicrobial properties of the E. gerardiana plant extract. Notably, these nanoparticles exhibited the most significant effect on E. coli and the least on 5. salivaris, with MIC value of 125 and 2000 μg/ml, respectively. Furthermore, they inhibited C. albicans growth at a concentration of 62.5 μg/ml. An assessment of the antioxidant properties of EG@AgNPs indicated a significant increase in antioxidant activity. Conclusion: The E. gerardiana plant extract has emerged as a promising option for silver nano-particle synthesis. These nanoparticles have been found to exhibit potent antimicrobial properties against Gram-positive and Gram-negative bacterial species, as well as C. albicans. Additionally, they have demonstrated antioxidant properties.
      原文链接:
    • NETL
    • NSTL
    • Bentham Science Publ Ltd

    Strengthening Effect of Thalidomide Combined with an Anti-PD1 Antibody on Enhancing Immunity for Lung Cancer Therapy

    Qing LiuZu-Chian ChiangXiangqian ZhaoDongya Cui...
    2724-2737页
    查看更多>>摘要：Objectives: Combining immune checkpoint inhibitors and antiangiogenic agents offers a promising strategy to counteract the cooperative promotion of solid tumor growth by immune checkpoints and intratumoral angiogenesis. Methods: We investigated the potential of thalidomide (THD) and anti-PD-1 antibody (PD-1 mAb) in suppressing tumor growth, enhancing immunity, and inhibiting angiogenesis. Results: THD exhibited regulatory effects on PD-1 in CD4~+ T cells and PD-L1 in cancer cells, along with tumor growth inhibition in A549 and Lewis lung carcinoma (LLC) cell lines. Combined with PD-1 mAb, THD increased intracellular IL-2 and IFN-γ expression in CD4~+ T cells, enhanced granzyme (Gzm-B) expression in peripheral blood mononuclear cells (PBMCs), and reduced TNF-a expression in CD4~+ T cells. In C57BL/6 mice, THD plus PD-1 mAb decreased LLC-derived lung tumor weight and volume, boosted CD8~+ T cell infiltration in tumors, and reduced CD34~+ intratumoral microvessel density. Conclusion: This study highlights THD's role in modifying the tumor microenvironment to enhance PD-1 mAb efficacy, proposing a clinically feasible approach for improving PD-1 mAb treatment outcomes.
      原文链接:
    • NETL
    • NSTL
    • Bentham Science Publ Ltd

    Circ-LRP6 Inhibits the Development and Progression of AAA Via miR-29a-3p/HIF-1α Axis

    Fang WangZhijian SunWenke YanHaiqing Wang...
    2738-2752页
    查看更多>>摘要：Background: At present, the research on the potential molecular mechanism of abdominal aortic aneurysm (AAA) is limited, which hinders the treatment of aneurysm and the development of drugs. CircRNA has been identified as a potential therapeutic target for diagnostic biomarkers in a variety of diseases. The purpose of this study was to explore the molecular mechanism of circLRP6 in AAA and to provide a theoretical basis for further clinical optimization of treatment. Methods: The animal model and cell model of AAA were constructed, and the circLRP6 expression was verified by in situ hybridization and qRT-PCR. The effect of circLRP6 on cell viability was determined using CCK-8 and BrdU. The effects of circLRP6 on the cell cycle and apoptosis were determined by flow cytometry. In addition, the interaction of circLRP6 with miR-29a-3p and HIF-1α was verified by the luciferase reporter gene and RIP. HIF-1α or caspase 3 expression was detected by immunofluorescence or western blot analysis. Results: Our previous results showed that the circLRP6 had reduced expression in AAA, and its overexpression significantly inhibited Angll-induced hAoSMC cell viability. In addition, bioin-formatics prediction showed that there was a binding site between miR-29a-3p and circLRP6, showing a negative regulatory relationship in hAoSMC. In addition, the results of the luciferase reporter gene and RIP showed that the circLRP6 interacted with HIF-1α, and achieved effective treatment of AAA by inhibiting the miR-29a-3p/HIF-1α. Conclusion: CircLRP6 effectively inhibited the development of AAA by inhibiting the miR-29a-3p/HIF-1α, providing a theoretical basis for further clinical optimization of treatment.
      原文链接:
    • NETL
    • NSTL
    • Bentham Science Publ Ltd