Abstract
Background: Colorectal cancer (CRC) is among the most fatal types of cancer. An active targeting delivery system that specifically interacts with CRC cells could improve the therapy's outcomes. Herein, Cetuximab single-chain fragment variable antibody (scFv) frag- . ments were conjugated to the surface of 5-FU encapsulated chitosan nanoparticles (CS NPs) to develop an effective therapeutic platform (scFv-CS/5-FU NPs). Methods: CS/5-FU NPs were synthesized using a special fluidic system. Encapsulation efficiency (EE), loading capacity (LC), and the drug release profile of the particles were determined. scFv fragments were produced recombinantly and tailored on the surface of CS/5-FU NPs. The physicochemical features of scFv-CS/5-FU NPs were also characterized. MTT and flow cytome-try assay investigated the toxicity effect of scFv-CS/5-FU NPs on the HCT116 cell line. Results: CS/5-FU NPs had a homogenous spherical shape. They possessed sustainable drug-release behavior. The produced scFv-CS/5-FU NPs were also spherical. scFv-CS/5-FU NPs significantly decreased the viability of cancerous cells in a dose-dependent manner and induced apoptosis in 97.97% of targeted cells. Conclusion: scFv-CS/5-FU NPs showed remarkable anti-CRC activity. This novel targeting delivery system reduced the effective dose of 5-FU which is of vital importance to decrease the devastating side effects of chemotherapy.
NETL
NSTL
Bentham Science Publ Ltd