首页|ADENOSINE TRIPHOSPHATE-BINDING CASSETTE TRANSPORTERS ARE NOT INVOLVED IN THE DETOXIFICATION OF AZADIRACHTA INDICA EXTRACTS IN ANOPHELES STEPHENSI LARVAE

ADENOSINE TRIPHOSPHATE-BINDING CASSETTE TRANSPORTERS ARE NOT INVOLVED IN THE DETOXIFICATION OF AZADIRACHTA INDICA EXTRACTS IN ANOPHELES STEPHENSI LARVAE

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Detoxifying pathways of mosquitoes against the neem (Azadirachta indica) extracts are still unclear. The aim of the present study was to investigate the role of adenosine triphosphate-binding cassette (ABC) transporters in this process in Anopheles stephensi, one of the main malaria vectors in southern Asia. Third-stage larvae of An. stephensi were fed with fish food alone or in combination with neem extract at 0.5%, 1%, 5%, and 10%. Six ABC-transporter genes from 3 different subfamilies (B, C, and G) were analyzed to assess their relative expression compared with controls. A bioassay was also performed to assess larval mortality rate at different concentrations and in combination with verapamil, an ABC-transporter inhibitor. No significant variation in the expression levels of any transporter belonging to the B, C, and G subfamilies was detected. Furthermore, the use of verapamil did not induce an increase in mortality at any of the tested neem extract concentrations, indicating that ABC transporters are not involved in the detoxification of neem extracts in An. stephensi larvae.

Detoxificationmosquito defensesnatural insecticidesneem treevector control

Ferrari, Marco、Negri, Agata、Romeo, Claudia、Boccazzi, Ilaria Varotto、Nodari, Riccardo、Habluetzel, Annette、Molteni, Giorgio、Corbett, Yolanda

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Texas Biomed Res Inst, 8715 W Milirary Dr, San Antonio, TX 78227 USA

Univ Roma La Sapienza, Dept Environm Biol, Via Cesare de Lollis 21, I-00185 Rome, Italy

Univ Milan, Dept Vet Med, Via Giovanni Celoria 10, I-20133 Milan, Italy

Univ Milan, Dept Biosci, Via Giovanni Celoria 26, I-20133 Milan, Italy

Univ Camerino, Sch Pharm, Via Madonna Carceri 9, I-62032 Camerino, Italy

Univ Milan, Dept Chem, Via Camillo Golgi 19, I-20133 Milan, Italy

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2018

Journal of the American Mosquito Control Association.

Journal of the American Mosquito Control Association.

ISSN：8756-971X
年,卷(期)：2018.34(4)