Biological trace element research.2022，Vol.200Issue(9) ：13.DOI:10.1007/s12011-021-02986-7

DNA Damage and Proteomic Profile Changes in Rat Salivary Glands After Chronic Exposure to Inorganic Mercury

Rabelo Buzalaf, Marilia Afonso Correa de Oliveira, Edivaldo Herculano Crespo-Lopez, Maria Elena Lima, Rafael Rodrigues Braganca Aragao, Walessa Alana Bittencourt, Leonardo Oliveira de Oliveira Lima, Leidiane Alencar Caldas de Souza, Michel Platini Nogueira, Lygia Sega Dionizio, Aline
Biological trace element research.2022，Vol.200Issue(9) ：13.DOI:10.1007/s12011-021-02986-7
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  • NSTL
  • Springer Nature

DNA Damage and Proteomic Profile Changes in Rat Salivary Glands After Chronic Exposure to Inorganic Mercury

Rabelo Buzalaf, Marilia Afonso 1Correa de Oliveira, Edivaldo Herculano 2Crespo-Lopez, Maria Elena 3Lima, Rafael Rodrigues 4Braganca Aragao, Walessa Alana 4Bittencourt, Leonardo Oliveira 4de Oliveira Lima, Leidiane Alencar 4Caldas de Souza, Michel Platini 5Nogueira, Lygia Sega 2Dionizio, Aline1
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作者信息

  • 1. Bauru Sch Dent,Univ Sao Paulo
  • 2. Environm Sect,Evandro Chagas Inst
  • 3. Inst Biol Sci,Fed Univ Para
  • 4. Universidade Federal do Para,Fed Univ Para
  • 5. Sect Parasitol,Evandro Chagas Inst
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Abstract

Mercury (Hg) is a toxic metal that became a public health problem due to environmental contamination caused by anthropogenic activity. In this sense, oral homeostasis can undergo changes due to the toxic effects of metal on the salivary glands. Therefore, our objective was to investigate the proteomic and genotoxic changes in salivary glands after exposure to inorganic mercury (IHg). Forty Wistar rats that were divided into a control group, which received distilled water, and an exposed group, which received 0.375 mg/kg of mercury chloride for 45 days via orogastric gavage. After that, the animals were euthanized, and the parotid and submandibular glands were collected for analysis of the genotoxic effects, using the comet assay and proteome global profile assessment. The results showed that IHg promoted damage to cellular DNA associated with proteomic changes that showed events such as oxidative stress, mitochondrial dysfunction, changes in the cytoskeleton, and apoptosis. Therefore, these findings show a profile of molecular changes due to the interactions of IHg with several proteins and mechanisms inherent to the cell, which consequently may result in dysfunction of the salivary glands and impaired homeostasis of the oral cavity.

Key words

Salivary glands/Parotid gland/Submandibular gland/Mercury chloride/Proteomics/DNA damage/FIBRILLARY ACIDIC PROTEIN/OXIDATIVE STRESS/CHRONIC INTOXICATION/CELL-DEATH/CHLORIDE/GENOTOXICITY/GLUTATHIONE/MECHANISMS/EXPRESSION

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出版年

2022
Biological trace element research.

Biological trace element research.

ISSN：0163-4984
参考文献量80
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