首页|Self-compatibility of 'Zaohuang' loquat is attributed to the lack of a 52 bp fragment in the S-10-RNase promoter
Self-compatibility of 'Zaohuang' loquat is attributed to the lack of a 52 bp fragment in the S-10-RNase promoter
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NSTL
Elsevier
Loquat (Eriobotrya japonica Lindl.) is a typical gametophytic self-incompatible (GSI) subtropical evergreen fruit tree, but there are also some self-compatible varieties. In this study, the fruit set rate of 'Zaohuang ((SS10)-S-2)' and 'Baiyu ((SS10)-S-2)' loquats were investigated after self-pollination and cross-pollination, respectively. The analysis demonstrated that 'Zaohuang' ('ZH') was self-compatible (SC) and 'Baiyu' ('BY') was self-incompatible (SI), while they were cross-compatible. The expression levels of S-RNase in the pistils of 'ZH' and 'BY' were analyzed by qPCR, indicating that the expression of S-2-RNase was similar in the two cultivars, but the expression of S-10-RNase in the pistils of 'ZH' was significantly lower than that in 'BY'. In addition, cloning of the full-length open reading frame (ORF) sequence and promoter of S-10-RNase in 'ZH' and 'BY' by TAIL-PCR showed that the fulllength ORF sequence of S-10-RNase was completely consistent in two loquat cultivars. However, the promoter of S-10-RNase in 'ZH' (ZH-S-10-RNase-P) lacks 52 bp fragment comparing with that in 'BY' (BY-S-10-RNase-P). The deletion fragment was located at 1755 bp upstream to the start code ATG of S-10-RNase, containing a TGACGmotif and a CGTCA-motif. To detect the activities of ZH-S-10-RNase-P and BY-S-10-RNase-P, we constructed ZHS(10)-RNase-P::GFP and BY-S-10--RNase-P::GFP plasmids and introduced them into tobacco pistils by transient expression method. The fluorescence of GFP was much lower in ZH-S-10--RNase-P::GFP transformed tobacco pistils than that in BY-S-10--RNase-P::GFP transformed pistils. These results indicated that the 52 bp deletion in the promoter of S-10-RNase results in reducing the expression of S-10--RNase and eventually leads to self-compatibility of 'ZH' loquat. Our research provided a new direction for screening loquat SC varieties with S-10-RNase promoter.
NSTL
Elsevier
