首页|Metabolomics combined with physiological and transcriptomic analyses reveal regulatory features associated with blueberry growth in different soilless substrates
Metabolomics combined with physiological and transcriptomic analyses reveal regulatory features associated with blueberry growth in different soilless substrates
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NSTL
Elsevier
The objective of this study was to evaluate the effects of soilless substrate mixtures on blueberry seedling growth under greenhouse conditions and to determine the optimal growth media for soilless blueberry (Vaccinium australe) cultivation. Trials were carried out with two-year-old blueberry seedlings from April to August 2021. Three mixed substrates (PB: peat and bark (1:1, v/v), PR: peat and rice husk (1:1, v/v), and BR: bark and rice husk (1:1, v/v)) were investigated. The results showed that the plant height, stem diameter and chlorophyll content were much higher in PB and PR than in BR. The PB and PR substrates triggered antioxidant system activity and exerted marked positive effects on blueberry seedling growth. The total flavonoid content was greatly increased in the PB group compared with the other two groups. Most element contents were greatly increased with BR compared with PB and PR. Furthermore, metabolomic and transcriptomic analyses revealed the critical responses of metabolic pathways to different substrate treatments in blueberry leaves collected in June. Overall, the metabolomic data revealed 757 metabolites with decreased levels and 1383 metabolites with increased levels. The levels of multiple prenol lipids, flavonoids, organooxygen compounds, carboxylic acids and derivatives were changed. "Flavonoid biosynthesis" was the most significantly enriched pathway and may be closely related to blueberry growth. Integrated transcriptomic and metabolomic data suggested that the expression of 4 DEGs, VcPAL, VcHCT, VcF14G24.3, and VcCHS, was strongly correlated with flavonoid biosynthesis. This work provides insights for elucidating the mechanisms that mediate the growth adaptability of blueberry cv. 'Zhaixuan 7' in different substrates.
NSTL
Elsevier
