A quantum dot microspheres-based highly specific and sensitive three-dimensional microarray for multiplexed detection of inflammatory factors

Lv, Yanbing ¹Xu, Hongke ²Wu, Ruili ¹Xu, Yanxia ¹Li, Ning ¹Li, Jinjie ¹Shen, Huaibin ¹Ma, Hongwei ²Guo, Fang ³Li, Lin Song¹

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作者信息

1. Henan Univ
2. Chinese Acad Sci
3. Liaoning Univ
折叠

Abstract

The development trend of in vitro diagnostics is to obtain various biological information from a sample at extremely low concentration and volume, which has promoted its progress in accurate and sensitive multiplexed detection. Here, we developed a single color quantum dot (QD) based three-dimensional (3D) structure matrix microarray and conducted the detection of two inflammatory factors (C-reactive protein (CRP) and serum amyloid A (SAA)) by a self-built fluorescence detection system. This strategy increased detection sensitivity by immobilizing the antibody specifically on the 3D substrate because it captured more than about 7 times of 'effective' antibodies compared to the two-dimensional (2D) plane. Compared to the dual QDs-2D fluorescence-linked immunosorbent assay, the limit of detection (LOD) of 3D microarray based on QDs modified with amphiphilic polymers has been further improved to 0.11 ng ml(-1) for SAA assay and to 0.16 ng ml(-1) for CRP assay, respectively. By using QD microspheres (SiO2@QDs@SiO2-COOH, containing approximately 200-300 hydrophobic QDs on per SiO2 sphere) as fluorescent labels, the LOD for CRP and SAA of 3D microarray reached as high as 15 pg ml(-1) and 86 pg ml(-1), and the sensitivity was further improved by 28-fold and 425-fold, respectively. Because of its excellent performance, this QD microspheres-based 3D microarray has great application potential for highly sensitive and multiplexed quantitative detection of other biomarkers, small molecules, and antibiotic residues in biomedicine and food safety.

Key words

aqueous quantum dot/SiO2@QDs@SiO2-COOH microspheres/3D structure microarray/multiplexed detection/inflammatory factors/IN-VITRO DIAGNOSTICS/CHEMILUMINESCENCE IMMUNOASSAY/QUANTITATIVE DETECTION/THICK-SHELL/PROTEIN/BIOSENSOR/ORIENTATION/ASSAYS

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出版年

2021

Nanotechnology

EISCI

ISSN：0957-4484

被引量5

参考文献量48

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