首页|FaLEC2 repressing FaLOX2 promoter involved in the metabolism of LOX-derived volatiles during strawberry ripening
FaLEC2 repressing FaLOX2 promoter involved in the metabolism of LOX-derived volatiles during strawberry ripening
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
? 2022Lipoxygenase (LOX) pathway is the key and the most important pathway for the metabolism of fruit volatile. However, the transcription mechanism of the LOX pathway is still obscure in non-climacteric fruit. In order to identify the transcription factor involved in LOX-derived volatile metabolism, RNA sequencing and metabolome were used to profile strawberry (Fragaria × ananassa Duch.). Correlation analysis shows that the expression of FaLOX2 was negatively correlated with the hexanal content (r = ?0.985). The RY element that specifically bound by the B3 domain transcription factors, are found in FaLOX2 promoter. B3 domain-containing transcription factors including LEAFY COTYLEDON2 (LEC2)–ABI3–VAL (LAV, 3), RELATED TO ABI3 and VP1 (RAV, 18), AUXIN RESPONSE FACTOR (ARF, 13), and REPRODUCTIVE MERISTEM (REM, 9) are identified. It is noticed that FaLEC2 expression is negatively correlated with (E,Z)-2,6-nonadienal content (r = ?1.000). Yeast one-hybrid analysis and dual-luciferase analysis suggest that FaLEC2 transcriptionally represses the activity of FaLOX2 promoter. Moreover, FaLEC2 expression level is negatively correlated with FaLOX2 expression (r = ?0.956). Here, our demonstration that FaLEC2 trans-represses the activity of the FaLOX2 promoter provides evidence that FaLEC2 directly controls a transcriptional program for LOX-derived volatile metabolism in strawberries.
College of Biosystems Engineering and Food Science Fuli Institute of Food Science Key Laboratory of Agro-Products Postharvest Handling Ministry of Agriculture and Rural Affairs Zhejiang University
College of Food Science and Engineering Henan University of Technology
NSTL
Elsevier
