首页|Yeast 2-Cys peroxiredoxin Tsa1 protects cells from DNA damage-induced reactive oxygen species through peroxidase activity
Yeast 2-Cys peroxiredoxin Tsa1 protects cells from DNA damage-induced reactive oxygen species through peroxidase activity
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NSTL
Korean Soc Applied Biological Chemistry
Peroxiredoxins (Prxs) belong to a family of thiol-specific peroxidases that scavenge reactive oxygen species (ROS). Yeast Prx thiol-specific antioxidant (Tsa1) protects cells from oxidative stress and maintains genome stability. In this study, we investigated the role of Tsa1 in the response to DNA damage. We observed that Tsa1 among all five Prxs of yeast is the most responsive in cell viability and mutation rate assays. The Delta tsa1 mutant is hypersensitive to DNA-damaging agents such as hydroxyurea, methyl methane sulfonate, and camptothecin. The Delta tsa1 cells lead to reduced cell viability and cell growth after DNA damage. The Delta tsa1-expressing wild-type Tsa1 rescues the sensitivity of Delta tsa1 cells to DNA damage, but not in Delta tsa1 cells carrying the active-site mutant Tsa1-C47S. We found that the level of ROS in Delta tsa1 cells was increased more than in wild-type cells by DNA damage. Tsa1 completely restored the production of DNA damage-induced ROS in Delta tsa1 cells, but not Tsa1-C47S. Finally, DNA damage-induced ROS resulted in a switch of Tsa1 from a low-molecular-weight to a high-molecular-weight complex. Taken together, these findings suggest that Tsa1 protects yeast cells from DNA damage by regulating the homeostasis of ROS through peroxidase activity.
DNA damageHypersensitivityPeroxidase activityPeroxiredoxinReactive oxygen speciesSaccharomyces cerevisiae
Jang, Ho Hee、Lee, Sang Yeol、Cui, Jun
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Gil Hosp, Gachon Med Res Inst, Inchon 405760, South Korea
Gyeongsang Natl Univ, Div Appl Life Sci, Plant Mol Biol & Biotechnol Res Ctr, Jinju 660701, South Korea
Gachon Univ, Lee Gil Ya Canc & Diabet Inst, Grad Sch Med, Dept Mol Med, Inchon 406840, South Korea
NSTL
Korean Soc Applied Biological Chemistry
