首页期刊导航|Journal of the Korean Society for Applied Biological Chemistry
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Journal of the Korean Society for Applied Biological Chemistry
Springer
Journal of the Korean Society for Applied Biological Chemistry

Springer

1738-2203

Journal of the Korean Society for Applied Biological Chemistry/Journal Journal of the Korean Society for Applied Biological ChemistrySCI
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    Yeast 2-Cys peroxiredoxin Tsa1 protects cells from DNA damage-induced reactive oxygen species through peroxidase activity

    Jang, Ho HeeLee, Sang YeolCui, Jun
    7页
    查看更多>>摘要：Peroxiredoxins (Prxs) belong to a family of thiol-specific peroxidases that scavenge reactive oxygen species (ROS). Yeast Prx thiol-specific antioxidant (Tsa1) protects cells from oxidative stress and maintains genome stability. In this study, we investigated the role of Tsa1 in the response to DNA damage. We observed that Tsa1 among all five Prxs of yeast is the most responsive in cell viability and mutation rate assays. The Delta tsa1 mutant is hypersensitive to DNA-damaging agents such as hydroxyurea, methyl methane sulfonate, and camptothecin. The Delta tsa1 cells lead to reduced cell viability and cell growth after DNA damage. The Delta tsa1-expressing wild-type Tsa1 rescues the sensitivity of Delta tsa1 cells to DNA damage, but not in Delta tsa1 cells carrying the active-site mutant Tsa1-C47S. We found that the level of ROS in Delta tsa1 cells was increased more than in wild-type cells by DNA damage. Tsa1 completely restored the production of DNA damage-induced ROS in Delta tsa1 cells, but not Tsa1-C47S. Finally, DNA damage-induced ROS resulted in a switch of Tsa1 from a low-molecular-weight to a high-molecular-weight complex. Taken together, these findings suggest that Tsa1 protects yeast cells from DNA damage by regulating the homeostasis of ROS through peroxidase activity.
      原文链接:
    • NSTL
    • Korean Soc Applied Biological Chemistry

    Flavonoids from Fragaria ananassa calyx and their antioxidant capacities

    Baek, Yoon-SuSong, Na-YoungNam, Tae-GyuKim, Dae-Ok...
    7页
    查看更多>>摘要：The calyx of Fragaria ananassa (Rosaceae) has not been investigated for its chemical constituents and biological activity. This study focused on the isolation of active metabolites from the calyx of this plant. Compounds from F. ananassa calyx were extracted in 80 % aqueous MeOH and the concentrated extract was successively partitioned into EtOAc, n-BuOH, and H2O fractions. Repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies afforded six purified flavonoids. On the basis of spectroscopic methods, including H-1, C-13 nuclear magnetic resonance spectrometry (NMR), DEPT, and 2D NMR (gCOSY, gHSQC, gHMBC), the flavonoids were identified as kaempferol (1), astragalin (2), quercetin (3), isoquercitrin (4), sexangularetin (5), and sinensin (6). All the flavonoids were isolated for the first time from the F. ananassa calyx in this study. The flavonoids were evaluated for radical-scavenging capacity 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid ammonium salt), 2,2-diphenyl-1-picrylhydrazyl, and oxygen radical absorbance capacity methods. In conclusion, quercetin (3) consistently showed the highest ABTS, DPPH, and ORAC radical-scavenging activities compared with the other isolated flavonoids.
      原文链接:
    • NSTL
    • Korean Soc Applied Biological Chemistry

    Effect of genetically modified rice producing resveratrol on the soil microbial communities

    Sohn, Soo-InOh, Young-JuKim, Byung-YongKweon, Soon-Jong...
    11页
    查看更多>>摘要：Cultivation of genetically modified (GM) crops has rapidly increased in the global agricultural area. However, cultivation of GM crops in the field evoked the concern of the possibility of unintentional consequences from transgenic plant into environment. In our present study, we have assessed the effects of RS526, GM rice producing resveratrol on the surrounding soil microbial community. The effects of RS526 on the soil microbial community in its field of growth were assessed using a conventional culture technique and culture-independent molecular methods. Three replicate field plots were planted with single GM rice and a non-GM counterpart, Dongjin. The soil microbial communities around these plants were compared using colony counting, denaturing gradient gel electrophoresis (DGGE), pyrosequencing analysis, and community-level physiological profiling during the growing periods. The bacterial, fungal, and actinomycetes population densities of the RS526 soils were found to be within the range of those of the non-GM rice cultivar. The DGGE banding patterns of the GM and non-GM soils were also similar, suggesting that the bacterial community structures were stable within a given month and were unaffected by the presence of a GM plant. The data obtained from pyrosequencing analysis showed that the bacterial community distribution at the phylum level were highly similar to DGGE patterns between two tested groups. The substrate utilization pattern of RS526 and Dongjin rice soil was quite similar between each sampling time. These results indicate that soil microbial communities are not significantly affected by the cultivation of RS526 within the experimental time frame.
      原文链接:
    • NSTL
    • Korean Soc Applied Biological Chemistry

    Recombinant expression of a novel antimicrobial peptide consisting of human alpha-defensin 5 and Mytilus coruscus mytilin-1 in Escherichia coli

    Shan, YingDong, YingJiang, Dong
    6页
    查看更多>>摘要：Antibiotic peptides are a battery of broad-spectrum antibacterial cationic polypeptides widely distributed in the plant and animal kingdoms. Among them, the human defensins are the first line of defense against pathogens and Mytilin, which is isolated from mussel serum, plays a key role in the mussel defense system. The antibacterial activity of these two peptides is generally ascribed to their overall positive charge, which enables them to disrupt bacterial membrane integrity and function. The aim of this study was to develop an effective method for the biosynthesis of a fusion peptide containing human alpha-defensin 5 (HD5) and Mytilin-1 in Escherichia coli to improve the antimicrobial activity. The individual HD5 and Mytilin-1 peptides were also synthesized for comparison with the fusion peptide. All the peptides, expressed as soluble fusions with the peptide thioredoxin, were isolated using a three-step purification strategy involving nickel-Sepharose chromatography, enterokinase cleavage, and cationic exchange chromatography. The identity of the peptides was confirmed by SDS-PAGE. Antimicrobial activity assays demonstrated that all the recombinant peptides had strong bactericidal properties and that the HD5 and Mytilin-1 fusion protein displayed higher activity against E. coli and Staphylococcus aureus. The results of this study provide a platform for the development of novel cationic peptides for biological studies.
      原文链接:
    • NSTL
    • Korean Soc Applied Biological Chemistry

    Safety and biotechnological properties of Enterococcus faecalis and Enterococcus faecium isolates from Meju

    Jeong, MiranJeong, Do-WonLee, Jong-Hoon
    8页
    查看更多>>摘要：We assessed the safety and biotechnological properties of 17 Enterococcus faecalis and 18 Enterococcus faecium strains isolated from Meju to select starter candidates for quality Meju production. Minimum inhibitory concentration assays showed that all strains were susceptible to ampicillin, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, penicillin G, and tetracycline, as judged by the breakpoint values defined at the species level by the European Committee on Antimicrobial Susceptibility Testing, whereas two E. faecalis strains were resistant to vancomycin, which could be ascribed to acquired resistance. None of the strains exhibited alpha- or beta-hemolytic activities or biofilm formation. Both species showed similar levels of biogenic amine production, and noticeable amounts of tyramine (3397.4 +/- A 172.4 ppm) were produced by all strains in the presence of precursors (histidine, lysine, ornithine, and tyrosine). All strains exhibited protease, lipase, and acid production, with decreased activity observed with increasing NaCl concentrations. This study confirmed the necessity of antibiotic resistance screening of Enterococcus species for food production, with the identification of vancomycin-resistant strains from Meju.
      原文链接:
    • NSTL
    • Korean Soc Applied Biological Chemistry

    Distribution and dissipation of deltamethrin, dimethomorph, imidacloprid, mepanipyrim, and metalaxyl applied to chili peppers

    Kwon, Chan HyeokLee, Young Deuk
    9页
    查看更多>>摘要：Field trials were carried out to investigate the dissipation rates of pesticides as well as to measure the terminal residues in chili peppers. Deltamethrin, dimethomorph, imidacloprid, mepanipyrim, and metalaxyl were applied to chili peppers 15 days prior to harvest. Chili pepper samples were collected at specified intervals and were subjected to residue analysis and dislodgeable residue study. In most cases, initial residue levels were rapidly dissipated with time. The dissipation pattern of pesticide residues fits well to first-order kinetics. Biological half-lives, as calculated from regression curves, of most pesticides were estimated to be < 4 days. In the fast growing chili pepper, a dilution effect was considerably involved in the rate of dissipation. As a result, it was possible to predict the terminal residue at harvested crops from the initial residue level and declining curve of pesticides.
      原文链接:
    • NSTL
    • Korean Soc Applied Biological Chemistry

    Antimicrobial potential of Leuconostoc species against E-coli O157:H7 in ground meat

    Koo, Ok KyungKim, Seung MinKang, Sun-Hee
    8页
    查看更多>>摘要：Ground beef is risky by foodborne pathogens such as E. coli O157:H7 due to the cross-contamination during grinding. The objective of this study was to evaluate the antagonistic activities of Leuconostoc species isolated from ground beef product in order to limit the growth of E. coli O157:H7. While Leuconostoc has been known as spoilage bacteria, the Leuconostoc isolates showed antimicrobial activity on foodborne pathogens such as E. coli O157:H7, Salmonella, Staphylococcus aureus, Listeria monocytogenes, and meat-spoilage bacteria Brochothrix thermosphacta. Antimicrobial activity of cell-free supernatant (CFS) was evaluated by heat, enzyme, and pH adjustment and antagonistic activity by cell competitive growth. In addition, CFS concentration was screened for the presence of organic acids for the antimicrobial properties. Regulation of virulence genes was investigated by inhibition of locus of enterocyte effacement expression by CFS. The result showed that Leuconostoc isolates inhibited the growth of E. coli O157:H7 by active antimicrobial agent including organic acids and the growth of background microorganisms, and E. coli O157:H7 in ground beef was inhibited with the supplement of the CFS.
      原文链接:
    • NSTL
    • Korean Soc Applied Biological Chemistry

    Bioactive carbazole alkaloids from Alysicarpus ovalifolius (Schumach)

    Akala, HoseaMwinzi, PaulineOpiyo, Sylvia AwinoOchung', Angeline Atieno...
    8页
    查看更多>>摘要：Phytochemical and biological evaluation of the stem bark of Alysicarpus ovalifolius led to the isolation of three carbazole alkaloids identified as mohanimbine (1), koenimbine (2) and koenidine (3) along with quercetin 3-O-glucoside (4), kaempferol 7-O-glucoside (5), orientin (6), apigenin (7), quercetin (8), plumbagin (9) and stigmasterol (10). The structures of these compounds were elucidated using physical and spectroscopic methods as well as comparison with the literature data. Compound 3 showed strong activity against chloroquine-sensitive strain I (D6) and the multi-drug resistant Indochicha I (W2) of Plasmodium falciparum with IC50 values of 63.07 +/- 0.01 and 54.19 +/- 0.04 ng/mL, respectively. Compound 1 on the other hand exhibited moderate larvicidal against Anopheles gambiae larvae as well as antimicrobial activities against Candida albicans and gram-positive Staphylococcus aureus, respectively.
      原文链接:
    • NSTL
    • Korean Soc Applied Biological Chemistry

    Isolation and characterization of rhizomicrobial isolates for phosphate solubilization and indole acetic acid production

    Hussein, Khalid AbdallahJoo, Jin Ho
    9页
    查看更多>>摘要：Recently, public concerns regarding the use of agrochemicals have increased due to the environmental impacts and potential risks to human health. The application of beneficial microorganisms is a novel technology to improve plant health and productivity and has therefore been extensively studied as an alternative strategy for biocontrol. In our study, 122 microbial isolates were obtained from the rhizosphere of Panax ginseng and subsequently tested in vitro for phosphate solubilization and indole acetic acid (IAA) production. Pikovskaya's medium was used to estimate rhizomicrobial isolates to solubilize tricalcium phosphate [Ca-3 (PO4)(2)]. Among all the investigated strains, 82 % of rhizospheric fungi showed phosphate solubilization activity; however, only 57.1 % of the rhizobacteria isolates showed phosphate solubilization ability. For IAA production, 64.7 % of the tested rhizofungi isolates were able to produce the phytohormone; however, only 47.62 % of the rhizobacteria isolates exhibited IAA production. Among all investigated species, Pseudomonas fluorescence and Azotobacter chroococcum showed the highest phosphate solubility demonstrating 885.4 and 863.4 mu g mL(-1), respectively. Mucor sp. produced 42.3 mu g mL(-1) of IAA in Czapek's tryptophan medium, and the highest fungal species to solubilize the inorganic phosphate (237.5 mu g mL(-1)) was estimated by Penicillium sp. Rhizobacteria were more effective than rhizofungi in phosphate solubilization and IAA production. This study introduces some potent species in terms of phosphate solubilization and IAA production which could be likely to improve soils' quality and promote plant growth.
      原文链接:
    • NSTL
    • Korean Soc Applied Biological Chemistry

    Effects of ascorbic acid on alpha-L-arabinofuranosidase and alpha-L-arabinopyranosidase activities from Bifidobacterium longum RD47 and its application to whole cell bioconversion of ginsenoside

    Ku, SeockmoYou, Hyun JuPark, Myeong SooJi, Geun Eog...
    9页
    查看更多>>摘要：Bifidobacterium longum RD47 was cultured in 24 kinds of modified MRS broths containing various ingredients to select the most promising source that induces microbial enzymes. Among the various ingredients, ascorbic acid significantly enhanced alpha-l-arabinofuranosidase and alpha-l-arabinopyranosidase activities in Bifidobacterium longum RD47. Addition of 2 % ascorbic acid (w/v) to MRS showed the maximum enzyme activities. Both whole cell and disrupted cell homogenates showed efficient rho-nitrophenyl-beta-d-glucopyranoside and rho-nitrophenyl-beta-d-glucofuranoside hydrolysis activities. The initially enhanced alpha-l-arabinopyranosidase and alpha-l-arabinofuranosidase activities by ascorbic acid were maintained over the cell disruption process. The optimal pH of alpha-l-arabinofuranosidase and alpha-l-arabinopyranosidase was 5.0 and 7.0, respectively. Both enzymes showed the maximum activities at 40.0 A degrees C. Under the controlled condition using Bifidobacterium longum RD47, ginsenoside Rb2, and Rc were converted to ginsenoside Rd.
      原文链接:
    • NSTL
    • Korean Soc Applied Biological Chemistry